PXD054355

PXD054355 is an original dataset announced via ProteomeXchange.

Title	LC-MS/MS proteomic analysis of pharyngeal swabs and plasma from MHV-infected and uninfected mice
Description	Recent developments have broadened our perception of SARS-CoV-2, indicating its capability to affect the body systemically beyond its initial recognition as a mere respiratory pathogen. However, the pathways of its widespread are not well-understood. Employing a dual-modality approach, we integrated findings from a Murine Hepatitis Virus (MHV) infection model with corroborative clinical data to investigate the pervasive reach of Coronaviruses. The novel presence of viral particles within red blood cells (RBCs) was demonstrated via High Resolution Transmission Electron Microscopy, with computational modeling elucidating a potential heme-mediated viral entry mechanism via Spike protein affinity. Our data affirm viral localization in RBCs, suggesting heme moieties as facilitators for cellular invasion. Exacerbation of MHV pathology upon hemin administration, contrasted with chloroquine-mediated amelioration, underscoring a heme-centric pathway in disease progression. These observations extend the paradigm of Coronavirus pathogenicity to include hemoprotein interactions. This study casts new light on the systemic invasion capabilities of Coronaviruses, linking RBC hemoproteins with viral virulence. The modulation of disease severity through heme-interacting agents heralds a promising avenue for COVID-19 therapeutics. Our findings propose a paradigm shift in the treatment approach, leveraging the virus-heme interplay as a strategic hinge for intervention.
HostingRepository	PRIDE
AnnounceDate	2024-12-11
AnnouncementXML	Submission_2024-12-11_08:17:51.846.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alejandro Leyva
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: NCBITaxon:2697049;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-29 16:30:41	ID requested
⏵ 1	2024-12-11 08:17:53	announced

Toro A, Ar, é, valo AP, Pereira-G, ó, mez M, Sabater A, Zizzi EA, Perbolianachis P, Pascual G, Lage-Vickers S, P, ó, rfido JL, Achinelli I, Seniuk R, Bizzotto J, Sanchis P, Olivera A, Leyva A, Moreno P, Cost, á, bile A, Fajardo A, Carri, ó, n F, Fl, ó M, Olivero-Deibe N, Rodriguez F, Nin N, Anselmino N, Labanca E, Vazquez E, Cotignola J, Alonso DF, Valacco MP, Marti M, Gentile F, Cherkasov A, Crispo M, Moratorio G, Gueron G, Blood matters: the hematological signatures of Coronavirus infection. Cell Death Dis, 15(11):863(2024) [pubmed]

10.1038/s41419-024-07247-8;

submitter keyword: pharyngeal swabs,Mus musculus, MHV, plasma, LC-M/MS

Rosario Durán
contact affiliation	Analytical Biochemistry and Proteomics Unit, Institut Pasteur de Montevideo & Instituto de Investigaciones Biológicas Clemente Estable.
contact email	duran@pasteur.edu.uy
lab head
Alejandro Leyva
contact affiliation	Institut Pasteur de Montevideo
contact email	alejandrolp@pasteur.edu.uy
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD054355

PRIDE project URI

• PRIDE
  1. PXD054355
     1. Label: PRIDE project
     2. Name: LC-MS/MS proteomic analysis of pharyngeal swabs and plasma from MHV-infected and uninfected mice