PXD054348

PXD054348 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of trophoblasts reveals calcium involvement in syncytialisation and hormone production
Description	Endocrine hormones of the placenta are essential for the maintenance of pregnancy, and disturbances in trophoblast integrity are associated with pregnancy complications. Here, we combine human trophoblast cell cultures, hormone assays, antibody-based detection methods and high-resolution mass spectrometry analyses to assess changes in cellular processes during syncytialisation. We monitored the changes in protein expression profiles during trophoblast syncytialization in an unbiased manner and show that the expression of numerous proteins is strongly altered. Among them are enzymes of the glucocorticoid and sex hormones synthesis pathways such as CYP19A1, CYP11A1, FDX1, HSD11B2 and HSD17B1, whose expression is strongly induced by syncytialization. The production of β-hCG, progesterone and estradiol increases several-fold during syncytialization, while the secretion and synthesis of ß-hCG and the expression of several syncytiotrophoblast markers show a clear calcium dependence
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_23:31:23.866.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054348
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Claudia Fecher-Trost
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-29 08:15:51	ID requested
⏵ 1	2025-05-06 23:31:25	announced

10.1186/s12958-025-01362-7;

10.6019/PXD054348;

Gehl AL, Klawitter D, Wissenbach U, Cole M, Wesely C, L, ö, hr H, Weissgerber P, Sota A, Meyer MR, Fecher-Trost C, -hCG) production. Reprod Biol Endocrinol, 23(1):33(2025) [pubmed]

submitter keyword: syncytialisation, trophoblast,placenta, calcium, hormone synthesis, proteome

Claudia Fecher-Trost
contact affiliation	Claudia Fecher-Trost Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University, Buildings 46, D-66421 Homburg, Germany
contact email	claudia.fecher-trost@uks.eu
lab head
Claudia Fecher-Trost
contact affiliation	PZMS
contact email	claudia.fecher-trost@uks.eu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054348

PRIDE project URI

• PRIDE
  1. PXD054348
     1. Label: PRIDE project
     2. Name: Proteome profiling of trophoblasts reveals calcium involvement in syncytialisation and hormone production