PXD054309

PXD054309 is an original dataset announced via ProteomeXchange.

Title	Characterization and functional differentiation of colostrum, transition milk, and mature milk somatic cells proteomes in multiparous Murrah buffaloes
Description	The objective of the present study was to examine the proteome dynamics of somatic cells from colostrum to mature milk. Milk somatic cells are a mixture of mammary epithelial cells (MECs) and immune cells, secreted in milk during milking. Besides protecting the mammary gland, the milk somatic cells have a dual role of contributing to the newborn calf’s health and development and it is unclear how their cargo is altered during the initiation of lactation. Somatic cells were isolated by density gradient centrifugation method from milk obtained from buffaloes on postpartum day 1 (D1; colostrum), 4 (D4; early transitional), 7 (D7; late transitional), and 15 (D15; mature). Quantitative proteomic analysis by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) of homogenate D1, D4, D7, and D15 (n = 6), identified a total of 4429 proteins. 71 proteins were uniquely expressed on D1, while 10, 25 and 15 unique proteins respectively on D4, D7 and D15. Of the high-confidence proteins, 3949 were common among all 4 days of lactation. The significantly changed proteins from the colostrum to mature milk were analyzed using gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and their interactions were generated using the Search Tool for Retrieval of Interacting Genes/Proteins (STRING) database. Results indicated the up-regulated proteins across the days were involved in metabolic processes like PPAR signaling, biosynthesis of lipids, amino acids and cofactors, amino and nucleotide sugar metabolism, glycolysis, and peroxisome. The down-regulated proteins were involved in phospholipid efflux and triglyceride (TG) homeostasis, electrolyte balance, mineral absorption and associated hormone regulation, complement and coagulation cascades, and immune-related functions like negative regulation of cytokine production, antigen processing and presentation, and mRNA processing, etc. Our study provides comprehensive alterations in the milk somatic cell proteome. In conclusion, the changes in biological functions indicate both mammary function/metabolism and the specific developmental requirements during the initial phases of calf development.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:12:59.488.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Priyanka M. Kittur
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-28 12:36:21	ID requested
⏵ 1	2025-07-13 16:13:00	announced

10.1038/s41598-025-08433-0;

Kittur PM, Satheesan L, Gunturu NT, Somagond YM, Madhusoodan AP, Gandham RK, Alex R, Dang AK, Proteomic analysis of Buffalo milk somatic cells reveals metabolomic and immunological transitions during early lactation. Sci Rep, 15(1):22704(2025) [pubmed]

submitter keyword: milk somatic cells, proteomics, transitional milk,Murrah buffaloes, colostrum, mature milk, LC-MS/MS

Dr. Ajay Kumar Dang
contact affiliation	Principal Scientist and Head of the Division, Animal Physiology Division, ICAR-NDRI, Karnal-132 001, Haryana, India
contact email	rajadang@gmail.com
lab head
Priyanka M. Kittur
contact affiliation	Research Scholar, Lactation and Immuno-physiology Laboratory, ICAR-National Dairy Research Institute, Karnal, Haryana, Inida-132001
contact email	mkpriyanka1246@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054309
     1. Label: PRIDE project
     2. Name: Characterization and functional differentiation of colostrum, transition milk, and mature milk somatic cells proteomes in multiparous Murrah buffaloes