PXD054296

PXD054296 is an original dataset announced via ProteomeXchange.

Title	Re-localization of components of the guided entry of tail-anchored proteins targeting pathway to cytosolic foci upon glucose starvation
Description	Protein targeting is an essential step in the biogenesis of membrane proteins and the guided-entry of tail-anchored proteins (GET) pathway is a key targeting route to the endoplasmic reticulum. The adaptive response to cellular stress is multifaceted but a hallmark is the formation of membraneless organelles, which can act as sequestration sites for particular factors, allowing regulation of cellular processes during stress and/or facilitating efficient recovery upon stress alleviation. Upon glucose withdrawal, we observe that the Get3 ATPase of the GET pathway re-locates to dynamic cytosolic foci, here termed GET bodies, that also contain proteins of the GET pathway pre-targeting complex. Our data demonstrate that Get3 and the Get4-Get5 heterodimer play important roles in the assembly of these structures, and we further uncover the requirement of the TPR domain of Sgt2 for the GET body formation. Compositional analyses of GET bodies by enrichment coupled to mass spectrometry as well as high-throughput microscopy-based screening reveal a chaperone-rich core and the association of an inventory of proteins involved in metabolic processes. Temporal analyses of GET body assembly demonstrate the sequential recruitment of different chaperones and we discover the requirement of the co-chaperones Sis1 and Sti1 for maintaining the dynamic properties of these structures. As lack of Adh2, which converts ethanol to acetaldehyde in a reaction coupled to NADH production, impairs GET body disassembly, and the addition of NADH promotes GET body dissolution in vitro, our results suggest a mechanistic basis for the formation of GET bodies in response to the metabolic shift caused by glucose withdrawal.
HostingRepository	PRIDE
AnnounceDate	2025-02-25
AnnouncementXML	Submission_2025-02-25_10:01:32.474.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-27 09:51:52	ID requested
⏵ 1	2025-02-25 10:01:32	announced

10.1242/JCS.263616;

submitter keyword: GET pathway, membrane proteins

Christof Lenz
contact affiliation	University Medical Center Goettingen, Department of Clinical Chemistry, Core Facility Proteomics
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD054296

PRIDE project URI

• PRIDE
  1. PXD054296
     1. Label: PRIDE project
     2. Name: Re-localization of components of the guided entry of tail-anchored proteins targeting pathway to cytosolic foci upon glucose starvation