PXD054276

PXD054276 is an original dataset announced via ProteomeXchange.

Title	TCR-engineered T cells targeting a shared β-catenin mutation eradicate solid tumors in vivo
Description	CTNNB1/ ꞵ-catenin is mutated in 3.3% of solid cancers, with several recurrent gain-of-function mutations occurring early during cancer formation. This includes the shared driver mutation CTNNB1S37F, estimated to occur in >7000 new cancer cases in 2024 in the US. However, to offer attractive immunotherapy targets, neoantigens must be presented at sufficient levels on tumor cells to be recognized by T cells. Here, we identify two neopeptides encoded by CTNNB1S37F presented on the frequent HLA-A*02:01 and HLA-A*24:02 alleles, from cell lines naturally expressing the mutation and HLA-alleles. T-cell receptors (TCRs) specifically recognizing the mutant peptides were isolated from naïve healthy donor T cells. T cells re-directed with the CTNNB1S37F TCRs efficiently killed CTNNB1S37F+ cell lines in vitro, eradicated established tumors in an in vivo melanoma mouse model naturally expressing the mutation and prevented relapses during the >100-day follow-up period. TCR T cells targeting CTNNB1S37F could form basis for promising immunotherapy in solid cancers.
HostingRepository	PRIDE
AnnounceDate	2025-07-16
AnnouncementXML	Submission_2025-07-16_05:24:23.264.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	The Trung Tran
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-26 07:53:50	ID requested
1	2025-07-15 04:41:16	announced
⏵ 2	2025-07-16 05:24:23	announced	2025-07-16: Updated project metadata.

Dataset with its publication pending

submitter keyword: β-catenin, In Vivo, T cells, Immunotherapy

Johanna Olweus
contact affiliation	Head, Dept of Immunology, Institute for Cancer Research, Oslo University Hospital Radiumhospitalet, Ullernchausséen 70, 0379 Oslo, Norway
contact email	johanna.olweus@medisin.uio.no
lab head
The Trung Tran
contact affiliation	University of Oslo
contact email	thetrung1268@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/07/PXD054276

PRIDE project URI

• PRIDE
  1. PXD054276
     1. Label: PRIDE project
     2. Name: TCR-engineered T cells targeting a shared β-catenin mutation eradicate solid tumors in vivo