PXD054252

PXD054252 is an original dataset announced via ProteomeXchange.

Title	Divergence in resource acquisition strategies and drivers of Prochlorococcus and Synechococcus abundance in the subtropical North Atlantic Ocean
Description	Surface waters of the vast subtropical ocean gyres are poor in nutrients such as nitrogen (N), phosphorus (P), iron (Fe) and zinc (Zn). Ocean warming is predicted to intensify and expand these low nutrient gyres, but we have limited knowledge on how natural assemblages of marine microbes acquire nutrients. While detecting regions where the lack of N and/or Fe limits biological activity appears straightforward, it is problematic for P due to divergent results from different approaches used. Using natural zonal gradients in nutrient resources and biological activity, we investigated the nutrient acquisition strategies of Prochlorococcus and Synechococcus, with a specific focus on phosphorus since Fe and Zn may constrain the activity of the metalloenzyme, alkaline phosphatase, used by microbes to access dissolved organic phosphorus (DOP) when phosphate is scarce. Analyses of proteins indicative of nutrient acquisition and stress, termed ‘biomarkers’ alongside high-resolution biogeochemical measurements and nutrient bioassays concur on more intense phosphorus limitation in the western compared to the eastern subtropical Atlantic. However, we found sparse evidence of trace metal control of alkaline phosphatase by Fe or Zn or cobalt. Instead, zonal gradients as well as the bioassays involving the addition of Fe, Zn and DOP stimulated different responses in protein production in Prochlorococcus and Synechococcus, implying these picocyanobacteria may have different biomarkers indicative of nutrient stress, which has implications for the interpretation of trends in protein, as well as different strategies to acquire essential nutrients. The divergence in acquisition strategies alongside the asynchronous zonal gradients in Prochlorococcus and Synechococcus in the subtropical North Atlantic are likely driven by other processes such as nitrogen fixation, atmospheric and upwelled delivery of nutrients and competition for resources.
HostingRepository	PRIDE
AnnounceDate	2025-12-10
AnnouncementXML	Submission_2025-12-10_09:50:51.430.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054252
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Noelle Held
SpeciesList	scientific name: SAR11 cluster bacterium enrichment culture clone PSHTC-005; NCBI TaxID: NEWT:1125417; scientific name: Prochlorococcus; NCBI TaxID: NEWT:1218; scientific name: Synechococcus sp. WH 8011; NCBI TaxID: NEWT:110446;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-25 18:26:47	ID requested
⏵ 1	2025-12-10 09:50:51	announced

10.6019/PXD054252;

submitter keyword: nutrient, Metaproteomics,Ocean, Microbe

Noelle Held
contact affiliation	University of Southern California
contact email	nheld@usc.edu
lab head
Noelle Held
contact affiliation	University of Southern California
contact email	nheld@usc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054252
     1. Label: PRIDE project
     2. Name: Divergence in resource acquisition strategies and drivers of Prochlorococcus and Synechococcus abundance in the subtropical North Atlantic Ocean