PXD054250

PXD054250 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Affinity purification – mass spectrometry of AFF4 WT or S829/S831/3/5/8A mutant
Description	The S831 phosphosite on AFF4 lies within a disordered region adjacent to the ENL/AF9 binding site. We therefore hypothesized that phosphorylation may regulate protein:protein interactions within the Super Elongation Complex. To investigate this, HEK293T cells expressing RFP negative control, FLAG-tagged AFF4-WT or FLAG-tagged AFF4-A-mutant were stimulated with insulin for 30 min followed by immunoprecipitation and analysis of interacting proteins by LC-MS/MS.
HostingRepository	PRIDE
AnnounceDate	2025-12-16
AnnouncementXML	Submission_2025-12-15_21:13:29.367.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-25 15:31:55	ID requested
⏵ 1	2025-12-15 21:13:29	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: affinity purification,AFF4, interactome

submitter keyword: affinity purification,AFF4, interactome

Contact List

Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054250
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054250

PRIDE project URI

Repository Record List

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