PXD054229

PXD054229 is an original dataset announced via ProteomeXchange.

Title	Phosphorylation landscape on time-resolved SYR1-dependent systemin signaling pathway
Description	Systemin was identified in 1991 as the first plant signaling peptide, and is required for defense against insect herbivores and necrotrophic pathogens in tomato plants. Systemin was first conceived as a hormone-like, long-distance messenger mediating the activation of systemic defense responses far from the site of insect attack. It was later shown to rather act as a phytocytokine, amplifying the local wound response for production of downstream signals resulting in defense gene activation in distal tissues. Systemin perception and signaling rely on the systemin receptor SYR1. However, SYR1-dependent signaling, and how systemin signaling differs from other peptide-mediated signaling pathways, is largely unknown. Here we used a Solanum peruvianum suspension cell culture to analyze phospho-proteomic responses triggered by 10 nM systemin. Samples were analyzed in a time series of 0, 1, 2, 5, 15, and 45 min after systemin treatment. To identify systemin-specific events a mutant cell culture lacking the SYR1 systemin receptor was analyzed in parallel. The experiment was performed in six biological replicates. Samples numbering scheme: Genotype_TreatmentTimeReplicate(e.g.,WT_S53 corresponding to wild-type cells treated with systemin, for 5min, the 3rd biological replicates)
HostingRepository	PRIDE
AnnounceDate	2025-06-17
AnnouncementXML	Submission_2025-06-17_02:49:39.564.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rong Li
SpeciesList	scientific name: Solanum peruvianum; NCBI TaxID: 4082;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-25 05:18:45	ID requested
⏵ 1	2025-06-17 02:49:39	announced

10.1038/S41477-025-02040-7;

submitter keyword: signaling transduction, phosphorylaion,time course,systemin, SYR1

Andreas Schaller
contact affiliation	Department of Plant Physiology and Biochemistry, University of Hohenheim, 70599 Stuttgart, Germany
contact email	andreas.schaller@uni-hohenheim.de
lab head
Rong Li
contact affiliation	University of Hohenheim
contact email	rong.li@uni-hohenheim.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054229
     1. Label: PRIDE project
     2. Name: Phosphorylation landscape on time-resolved SYR1-dependent systemin signaling pathway