PXD054202 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | SLC17 transporters mediate renal excretion of Lac-Phe in mice and humans |
Description | N-lactoyl-phenylalanine (Lac-Phe) is a lactate-derived metabolite that suppresses food intake and body weight. Little is known about the mechanisms that mediate Lac-Phe transport across cell membranes. Here we identify SLC17A1 and SLC17A3, two kidney-restricted plasma membrane-localized solute carriers, as physiologic urine Lac-Phe transporters. In cell culture, SLC17A1/3 exhibit high Lac-Phe efflux activity. In humans, levels of Lac-Phe in urine exhibit a strong genetic association with the SLC17A1-4 locus. Urine Lac-Phe levels are also increased following a Wingate sprint test. In mice, genetic ablation of either SLC17A1 or SLC17A3 reduces urine Lac-Phe levels. Despite these differences, both knockout strains have normal blood Lac-Phe and body weights, demonstrating SLC17-dependent de-coupling of urine and plasma Lac-Phe pools. Together, these data establish SLC17A1/3 family members as the physiologic urine transporters for Lac-Phe and uncover a biochemical pathway for the renal excretion of this signaling metabolite. Our data do not exclude the involvement of other transporters in mediating Lac-Phe transport. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_02:50:41.700.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD054202 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Veronica Li |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-24 20:06:30 | ID requested | |
⏵ 1 | 2024-10-17 02:50:42 | announced | |
Publication List
10.1038/s41467-024-51174-3; |
Li VL, Xiao S, Schlosser P, Scherer N, Wiggenhorn AL, Spaas J, Tung AS, Karoly ED, K, รถ, ttgen A, Long JZ, SLC17A1/3 transporters mediate renal excretion of Lac-Phe in mice and humans. Nat Commun, 15(1):6895(2024) [pubmed] |
10.6019/PXD054202; |
Keyword List
submitter keyword: Kidney,Lac-Phe, Transporter, Urine |
Contact List
Jonathan Zhong Long |
contact affiliation | Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA. |
contact email | jzlong@stanford.edu |
lab head | |
Veronica Li |
contact affiliation | Stanford University |
contact email | ver0nica@stanford.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD054202 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054202
- Label: PRIDE project
- Name: SLC17 transporters mediate renal excretion of Lac-Phe in mice and humans