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PXD054189

PXD054189 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTheoretical assessment of indistinguishable peptides in mass spectrometry-based proteomics
DescriptionMass spectrometry-based proteomics has advanced with the integration of experimental and predicted spectral libraries, which have significantly improved peptide identification in complex search spaces. However, challenges persist in distinguishing some peptides with close retention times and nearly identical fragmentation patterns. In this study, we conducted a theoretical assessment to quantify the prevalence of indistinguishable peptides within the human canonical proteome and immunopeptidome using state-of-the-art retention time and spectrum prediction models. By quantifying the proportion of peptides posing challenges to unequivocal identification, we set the accessible portion within a given proteome, thus establishing the current boundaries of the field, and underscore the effectiveness of contemporary analytical methodologies in resolving the complexity of the human proteome and immunopeptidome via mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2024-10-11
AnnouncementXMLSubmission_2024-10-11_01:00:48.120.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEduard Sabidó
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-24 11:23:38ID requested
12024-10-11 01:00:58announced
Publication List
10.1021/acs.analchem.4c02803;
Elhamraoui Z, Borr, à, s E, Wilhelm M, Sabid, ó E, Theoretical Assessment of Indistinguishable Peptides in Mass Spectrometry-Based Proteomics. Anal Chem, 96(40):15829-15833(2024) [pubmed]
Keyword List
submitter keyword: Hela, proteomics, DIA
Contact List
Eduard Sabidó
contact affiliationCentre for Genomic Regulation, Universitat Pompeu Fabra
contact emaileduard.sabido@crg.cat
lab head
Eduard Sabidó
contact affiliationCentre de Regulació Genòmica
contact emaileduard.sabido@crg.cat
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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