PXD054188 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Functional characterisation of tumour suppressor PDCD4 reveals a previously undisclosed role in the control of cell adhesion |
| Description | PDCD4 is a multifunctional RNA binding protein which has tumour suppressor function. To more fully understand how dysregulation of this protein contributes to carcinogenesis we have carried out a comprehensive analysis of the role of PDCD4 in RNA metabolism in untransformed epithelial cells. We show that PDCD4 predominantly localises in the nucleus, where it interacts with proteins involved in a range of different RNA metabolic processes. We find that PDCD4 knockdown is associated with significant changes in either the expression or splicing of a number of transcripts, although it appears to have an indirect role in splicing. We identified the RNA targets of PDCD4 using iCLIP and observed an enrichment in binding to transcripts encoding cell adhesion and structural proteins. Consistent with these data, we show that PDCD4 acts as a general regulator of cell adhesion, which in a tumour setting would increase the metastatic potential of cells, and demonstrate that the nuclear localisation of PDCD4 is crucial in this process. Overall, the information obtained in untransformed cells provides new perspective for the role of PDCD4 as a tumour suppressor. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-08-18 |
| AnnouncementXML | Submission_2026-08-18_06:43:07.494.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sandip Kumar Patel |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; |
| ModificationList | iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-07-24 11:21:39 | ID requested | |
| 1 | 2024-07-31 03:07:31 | announced | |
| 2 | 2026-08-18 01:55:58 | announced | 2026-08-18: Updated project metadata. |
| ⏵ 3 | 2026-08-18 06:43:09 | announced | 2026-08-18: Updated project metadata. |
Publication List
| 10.1093/nar/gkag071; |
| Dezi V, Harvey RF, Smith T, Cole CH, Pizzinga M, Stoneley M, Garland GD, Horvilleur E, Kalmar L, Sawarkar R, Lilley KS, Willis AE, Functional characterisation of tumour suppressor PDCD4 reveals previously undisclosed role in the control of cell adhesion. Nucleic Acids Res, 54(3):(2026) [pubmed] |
Keyword List
| submitter keyword: RNA-binding,PDCD4, nucleus, cell adhesion, translation control |
Contact List
| Anne Willis |
|---|
| contact affiliation | MRC Toxicology Unit, University of Cambridge |
| contact email | tls36@cam.ac.uk |
| lab head | |
| Sandip Kumar Patel |
|---|
| contact affiliation | Proteomics Core Facility, MRC Toxicology Unit, University of Cambridge |
| contact email | proteomics@mrc-tox.cam.ac.uk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD054188 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054188
- Label: PRIDE project
- Name: Functional characterisation of tumour suppressor PDCD4 reveals a previously undisclosed role in the control of cell adhesion
