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PXD054183

PXD054183 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNuclear release of eIF1 globally increases stringency of start codon selection to preserve mitotic arrest physiology
DescriptionRegulated start codon selection has the potential to reshape the proteome through the differential production of uORFs, canonical proteins, and alternative translational isoforms. However, conditions under which start codon selection is altered remain poorly defined. Here, using transcriptome-wide translation initiation site profiling, we reveal a global increase in the stringency of start codon selection during mammalian mitosis. Low-efficiency initiation sites are preferentially repressed in mitosis, resulting in pervasive changes in the translation of thousands of start sites and their corresponding protein products. This increased stringency of start codon selection during mitosis results from increased associations between the 40S ribosome and the key regulator of start codon selection, eIF1. We find that increased eIF1-40S ribosome interactions during mitosis are mediated by the release of a nuclear pool of eIF1 upon nuclear envelope breakdown. Selectively depleting the nuclear pool of eIF1 eliminates the changes to translational stringency during mitosis, resulting in the altered synthesis of thousands of protein isoforms. In addition, preventing mitotic translational rewiring results in substantially increased cell death and decreased mitotic slippage in cells that experience a mitotic delay induced by anti-mitotic chemotherapeutics. Thus, cells globally control translation initiation stringency with critical roles during the mammalian cell cycle to preserve mitotic cell physiology.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_07:00:50.525.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD054183
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJimmy Ly
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-24 08:09:40ID requested
12024-10-07 10:52:05announced
22024-10-22 07:00:51announced2024-10-22: Updated project metadata.
Publication List
10.1101/2024.04.06.588385;
Ly J, Xiang K, Su KC, Sissoko GB, Bartel DP, Cheeseman IM, Nuclear release of eIF1 globally increases stringency of start-codon selection to preserve mitotic arrest physiology. bioRxiv, ():(2024) [pubmed]
10.6019/PXD054183;
Keyword List
submitter keyword: Translation, ribosomes, mitosis, cell cycle
Contact List
Iain Cheeseman
contact affiliationWhitehead Institute and MIT
contact emailicheese@wi.mit.edu
lab head
Jimmy Ly
contact affiliationPhD student
contact emailjimmyly@wi.mit.edu
dataset submitter
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Dataset FTP location
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