PXD054173
PXD054173 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | LINE-1 ORF1p co-immunoprecipitations analyzed by DDA and two DIA methods |
| Description | Using an anti-ORF1 monoclonal (clone 4H1; Milipore), four co-immunoprecipitations to enrich for LINE-1 ORF1p and its interactors as described in Di Stefano, L.H. et al. (https://doi.org/10.1007/978-1-0716-2883-6_12), from either HEK-293TLD (cells transfected with pMT646- containing a L1 sequence) or N2102Ep (embryonal carcinoma cells with endogenous expression of LINE-1) lysates were then analyzed in DDA, HRMS1, and Variable Window modes. Mouse polyclonal IgG were used as mock IPs as nonspecific binding controls. MMTS alkylated the cysteines. DDA and Variable Window experiments collected spectra for 60 minutes of the one hour run time. The HRMS1 experiments collected spectra for 90 minutes of a two hour run time. The UP000005640 reference proteome for Homo sapiens, including unreviewed sequences and reviewed isoforms was used as a search space (104,558 sequences) |
| HostingRepository | MassIVE |
| AnnounceDate | 2024-09-10 |
| AnnouncementXML | Submission_2024-09-10_03:03:08.958.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | David L. Tabb |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | L-cysteine methyl disulfide; Oxidation |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-07-24 02:39:33 | ID requested | |
| ⏵ 1 | 2024-09-10 03:03:09 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: co-immunoprecipitation, data-independent acquisition, LINE-1 |
Contact List
| John P. LaCava | |
|---|---|
| contact affiliation | University of Groningen |
| contact email | jlacava@rockefeller.edu |
| lab head | |
| David L. Tabb | |
| contact affiliation | University of Groningen |
| contact email | d.l.tabb@rug.nl |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/v08/MSV000095427/ |
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