PXD054172

PXD054172 is an original dataset announced via ProteomeXchange.

Title	Comprehensive Analysis of Blood Proteome Response to Necrotic Enteritis in Broiler Chicken
Description	Necrotic enteritis (NE) in broiler chickens, caused by the overgrowth of toxin-producing strains of Clostridium (C.) perfringens, results in the development of necrotic lesions, compromised intestinal health, and significant economic losses in poultry production. This study aims to analyze the blood proteome of broiler chickens affected by NE, providing insights into the host's response to the infection. Using MS/MS-based proteomics, blood plasma samples from broilers with necrotic lesions of different severity were analyzed and compared to healthy controls. A total of 412 proteins were identified, with 63 showing significant differences and (for some of those) correlating with disease severity. Gene Set Enrichment Analysis (GSEA) revealed that proteins affected by NE were predominantly associated with the immune and signaling processes and extracellular matrix (ECM) structures. Notably, regulated proteins were significantly involved in bioprocesses related to complement activation, acute phase reaction, proteolysis and humoral immune response. The findings suggest that the changes in plasma proteins in response to NE are driven by the host's intensified efforts to counteract the infection, demonstrating a.o. a notable reduction in peptides from ECM-related proteins in the blood of NE-affected birds. Overall, proteomics results underscored the attempts of the host to manage tissue damage and inflammation, indicating a coordinated effort to mitigate the pathogenic impact of C. perfringens. This study provides a deeper understanding of the host-pathogen interactions and potential targets for therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_22:33:58.423.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Svitlana Tretiak
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-24 02:24:13	ID requested
⏵ 1	2025-05-06 22:33:59	announced

10.1186/s13567-025-01519-7;

Tretiak S, Mendes Maia T, Rijsselaere T, Van Immerseel F, Ducatelle R, Impens F, Antonissen G, Comprehensive analysis of blood proteome response to necrotic enteritis in broiler chicken. Vet Res, 56(1):88(2025) [pubmed]

submitter keyword: ECM, broiler chicken, proteomics,Biomarkers, gut health, necrotic enteritis

Francis Impens
contact affiliation	VIB-UGent Center for Medical Biotechnology UGent Department of Biomolecular Medicine Technologiepark-Zwijnaarde 75 B9052 Gent Belgium
contact email	Francis.Impens@vib-ugent.be
lab head
Svitlana Tretiak
contact affiliation	Ph.D. student
contact email	svitlana.tretiak@ugent.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054172
     1. Label: PRIDE project
     2. Name: Comprehensive Analysis of Blood Proteome Response to Necrotic Enteritis in Broiler Chicken