PXD054169 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Insights into the role of VPS39 and its interaction with CP204L and A137R in ASFV infection |
Description | The African swine fever virus (ASFV) is a large and complex DNA virus that causes a highly le-thal disease in swine, for which no antiviral drugs or vaccines are currently available. Studying viral–host protein-protein interactions advances our understanding of the molecular mecha-nisms underlying viral replication and pathogenesis and can facilitate the discovery of antiviral therapeutics. In this study, we employed affinity tagging and purification mass spectrometry to characterize the interactome of VPS39, an important cellular factor during the early phase of ASFV replication. The interaction network of VPS39 revealed associations with mitochondrial proteins involved in membrane contact sites formation and cellular respiration. We show that ASFV proteins CP204L and A137R target VPS39 by interacting with its clathrin heavy chain func-tional domain. Furthermore, we elaborate on the potential mechanisms by which VPS39 may contribute to ASFV replication and prioritize interactions for further investigation into mito-chondrial protein function in the context of ASFV infection. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:58:59.299.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Katarzyna Dolata |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-24 00:32:36 | ID requested | |
1 | 2024-09-17 10:18:08 | announced | |
⏵ 2 | 2024-10-22 06:58:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: African swine fever virus |
protein-protein interaction |
virus-host interaction |
Contact List
Axel Karger |
contact affiliation | Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald-Insel Riem,Germany |
contact email | axel.karger@fli.de |
lab head | |
Katarzyna Dolata |
contact affiliation | Friedrich-Loeffler Institut |
contact email | katarzyna.dolata@fli.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054169
- Label: PRIDE project
- Name: Insights into the role of VPS39 and its interaction with CP204L and A137R in ASFV infection