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PXD054163

PXD054163 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMulti-component Toxicological Study on Rare Earth Upconversion Nanoparticles
DescriptionUCNPs@PEG was divided into three groups, 0.0 mg/mL, 0.25 mg/mL, 1.0 mg/mL, and each concentration was repeated for three groups. After co-culture with AML12 cells for 24h, the medium was removed by pipette gun, and sterilized PBS was added for washing 3 times. 300μ L RIPA cell lysate was added to the culture dish. The lysed cells were scraped off with sterile cell scraper, and the lysed cell solution was sucked into the cryopreservation tube.(TandemMassTags) Labeled protein for proteomic study. First extract the total protein in cells, then do concentration determination and SDS-PAGE detection, then use TMT labeled protein after protease hydrolysis, label samples after uniform mixing chromatography separation, and then LC-MS/MS analysis. Chromatographic reaction conditions: A phase H2O-FA (99.9: 0.1, v/v), Phase B ACN-H2O-FA (80: 19.9: 0.1, v/v/v), mass resolution 60000, scan charge-to-mass ratio range 350-1500, collision energy 32, resolution 45000, automatic gain control 2e5. Functional annotation analysis of identified proteins was carried out using common protein databases. Differential proteins were screened and GO, KEGG and interaction analysis were carried out respectively. Cluster thermomap and Veen analysis were used to analyze differential data.
HostingRepositoryiProX
AnnounceDate2024-07-23
AnnouncementXMLSubmission_2024-07-23_19:51:28.372.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJianjun Shen
SpeciesList scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-23 19:43:33ID requested
12024-07-23 19:51:28announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Rare Earth Upconversion Nanoprobes, toxicology, Multi-omics
Contact List
Changlong Jiang
contact affiliationHefei Institutes of Physical Science, Chinese Academy of Sciences
contact emailcljiang@iim.ac.cn
lab head
Jianjun Shen
contact affiliationHefei Institutes of Physical Science, Chinese Academy of Sciences
contact emailjianjunshen@mail.ustc.edu.cn
dataset submitter
Full Dataset Link List
iProX dataset URI