PXD054163
PXD054163 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Multi-component Toxicological Study on Rare Earth Upconversion Nanoparticles |
Description | UCNPs@PEG was divided into three groups, 0.0 mg/mL, 0.25 mg/mL, 1.0 mg/mL, and each concentration was repeated for three groups. After co-culture with AML12 cells for 24h, the medium was removed by pipette gun, and sterilized PBS was added for washing 3 times. 300μ L RIPA cell lysate was added to the culture dish. The lysed cells were scraped off with sterile cell scraper, and the lysed cell solution was sucked into the cryopreservation tube.(TandemMassTags) Labeled protein for proteomic study. First extract the total protein in cells, then do concentration determination and SDS-PAGE detection, then use TMT labeled protein after protease hydrolysis, label samples after uniform mixing chromatography separation, and then LC-MS/MS analysis. Chromatographic reaction conditions: A phase H2O-FA (99.9: 0.1, v/v), Phase B ACN-H2O-FA (80: 19.9: 0.1, v/v/v), mass resolution 60000, scan charge-to-mass ratio range 350-1500, collision energy 32, resolution 45000, automatic gain control 2e5. Functional annotation analysis of identified proteins was carried out using common protein databases. Differential proteins were screened and GO, KEGG and interaction analysis were carried out respectively. Cluster thermomap and Veen analysis were used to analyze differential data. |
HostingRepository | iProX |
AnnounceDate | 2024-07-23 |
AnnouncementXML | Submission_2024-07-23_19:51:28.372.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jianjun Shen |
SpeciesList | scientific name: Mus musculus; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-07-23 19:43:33 | ID requested | |
⏵ 1 | 2024-07-23 19:51:28 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Rare Earth Upconversion Nanoprobes, toxicology, Multi-omics |
Contact List
Changlong Jiang | |
---|---|
contact affiliation | Hefei Institutes of Physical Science, Chinese Academy of Sciences |
contact email | cljiang@iim.ac.cn |
lab head | |
Jianjun Shen | |
contact affiliation | Hefei Institutes of Physical Science, Chinese Academy of Sciences |
contact email | jianjunshen@mail.ustc.edu.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |