PXD054105

PXD054105 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Robust proteome profiling of cysteine-reactive fragments using label-free chemoproteomics - HEK293T - Global expression proteomics
Description	In this manuscript we describe our work on the development of a label-free chemoproteomics screening platform for cysteine reactive covalent fragments on a 96 well plate format. This platform profiles cysteine reactive fragments by competition with the hyper-reactive iodoacetamide desthiobiotin (IA-DTB) in cell lysates and live cells. We employ label free quantification and data independent acquisition (DIA) on an Evosep One – Bruker timsTOF Pro. In this submission we report this use of global proteomics to explore protein expression in HEK293T.
HostingRepository	PRIDE
AnnounceDate	2024-11-18
AnnouncementXML	Submission_2024-11-17_16:54:22.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	George Biggs
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-22 03:35:04	ID requested
⏵ 1	2024-11-17 16:54:22	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Data independant acquistion, label free quantification

submitter keyword: Data independant acquistion, label free quantification

Contact List

Jacob Bush
contact affiliation	Crick-GSK LinkLabs, UK
contact email	jacob.x.bush@gsk.com
lab head
George Biggs
contact affiliation	The Francis Crick Institute
contact email	george.biggs@crick.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD054105
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD054105

PRIDE project URI

Repository Record List

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