PXD054094

PXD054094 is an original dataset announced via ProteomeXchange.

Title	Plasma proteome sequencing for obese patients versus healthy populations contains two cohorts
Description	Added 8 M urea solution to the frozen plasma sample, quantified and enriched the low-abundance proteins were using the ProteoMiner™ Protein Enrichment Kit (Bio-Rad, Hercules, USA). Added triethylammonium bicarbonate buffer (TEAB) to a 100 μg sample to reach a final concentration of 100 mM, along with tris-(2-carboxyethyl) phosphine at a final concentration of 10 mM. Incubated this mixture at 37 ℃ for 60 min, and subsequently added iodoacetamide to a final concentration of 40 mM, reacted for 40 min at room temperature in the dark. After centrifuging for 20 min at 10,000 G, dissolved the precipitate in 100 μL of 100 mM TEAB, and digested the proteins with trypsin (m/m, 1:50) at 37 ℃ to obtain peptide segments, which were then dried in a vacuum centrifugal concentrator (Taicang Huamei Biochemical Instrument Factory, Taicang, China) and redissolved in a solution containing 1% trifluoroacetic acid. Desalted the peptide segments using a hydrophilic-lipophilic balance and quantified them following the kit instructions (Thermo Fisher Scientific, Waltham, USA) after drying in a vacuum centrifugal concentrator. Finally, dissolved these peptide segments in loading buffer for downstream analyses.
HostingRepository	iProX
AnnounceDate	2024-07-22
AnnouncementXML	Submission_2024-07-22_00:30:55.729.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yunan Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-22 00:20:16	ID requested
⏵ 1	2024-07-22 00:30:56	announced

Dataset with its publication pending

submitter keyword: obese

Guangrui Huang
contact affiliation	Beijing University of Chinese Medicine
contact email	hgr@bucm.edu.cn
lab head
Yunan Zhang
contact affiliation	Beijing University of Chinese Medicine
contact email	20210931173@bucm.edu.cn
dataset submitter

iProX dataset URI