PXD054042

PXD054042 is an original dataset announced via ProteomeXchange.

Title	Structural basis of respiratory complexes adaptation to cold temperatures
Description	In response to cold, mammals activate brown fat for respiratory-dependent thermogenesis reliant on the electron transport chain. Yet, the structural basis of respiratory complex adaptation to cold remains elusive. Herein we combined thermoregulatory physiology and cryo-EM to study endogenous respiratory supercomplexes exposed to different temperatures. A cold-induced conformation of CI:III2 (termed type 2) was identified with a ~25 rotation of CIII2 around its inter-dimer axis, shortening inter-complex Q exchange space, and exhibiting different catalytic states which favor electron transfer. Large-scale supercomplex simulations in lipid membrane reveal how unique lipid-protein arrangements stabilize type 2 complexes to enhance catalytic activity. Together, our cryo-EM studies, multiscale simulations and biochemical analyses unveil the mechanisms and dynamics of respiratory adaptation at the structural and energetic level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:00:20.153.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pere Puigserver
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-18 08:50:41	ID requested
1	2024-10-01 17:52:14	announced
⏵ 2	2024-10-22 07:00:20	announced	2024-10-22: Updated project metadata.

10.1016/J.CELL.2024.09.029;

submitter keyword: Obesity, Mitochondria, PPID, TOM70, PERK, Mitochondrial Transport, chaparone

Pere Puigserver
contact affiliation	Dana-Farber Cancer Institute, Department of Cancer Biology. Harvard Medical School, Department of Cell Biology.
contact email	pere_puigserver@dfci.harvard.edu
lab head
Pere Puigserver
contact affiliation	Dana-Farber Cancer Intitute, Harvard Medical School
contact email	pere_puigserver@dfci.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD054042

PRIDE project URI

• PRIDE
  1. PXD054042
     1. Label: PRIDE project
     2. Name: Structural basis of respiratory complexes adaptation to cold temperatures