PXD054033

PXD054033 is an original dataset announced via ProteomeXchange.

Title	OmpA controls order in the outer membrane and shares the mechanical load
Description	OmpA, a predominant outer membrane protein (OMP) in Gram-negative bacteria, plays a crucial role in maintaining the integrity and functionality of the bacterial outer membrane (OM). Despite its significance in virulence, adhesion, and OM stability, the specific role of OmpA has remained unclear since its discovery 50 years ago. In this study, we demonstrate that OmpA organizes the OMP lattice and mechanically connects it to the cell wall. Using atomic force microscopy, simulations, and microfluidics, we show that while the β-barrel domain of OmpA is essential for maintaining the permeability barrier, both the β-barrel and cell wall-binding domains are necessary to enhance the cell envelope's strength. We propose that OmpA integrates the compressive properties of the OMP lattice with the tensile strength of the cell wall, forming a mechanically robust composite that enhances overall envelope integrity. This coupling likely underpins the ability of the entire envelope to function as a cohesive and resilient structure, critical for the survival and pathogenicity of Gram-negative bacteria.
HostingRepository	PRIDE
AnnounceDate	2024-11-20
AnnouncementXML	Submission_2024-11-20_10:00:49.318.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex Johnson
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-18 03:02:12	ID requested
⏵ 1	2024-11-20 10:00:49	announced

Dataset with its publication pending

submitter keyword: Escherichia coli

Martin Wühr
contact affiliation	Princeton University
contact email	wuhr@princeton.edu
lab head
Alex Johnson
contact affiliation	Princeton University
contact email	aj10@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD054033

PRIDE project URI

• PRIDE
  1. PXD054033
     1. Label: PRIDE project
     2. Name: OmpA controls order in the outer membrane and shares the mechanical load