PXD054031

PXD054031 is an original dataset announced via ProteomeXchange.

Title	H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation – Dataset 1
Description	Ribosome biogenesis follows a hierarchical set of pre-ribosomal RNA (pre-RNA) folding and processing steps. The earliest, biochemically stable pre-ribosome is the 90S particle, which is assembled co-transcriptionally in the nucleolus. It is unclear how pre-rRNA, ribosomal proteins and assembly factors play together in the early stages of 90S maturation. Is has remained unclear, how the numerous snoRNAs, which guide pre-rRNA modification and folding while the nascent rRNA is flexible and hence accessible, are involved in pre-ribosomal maturation. Here, we identify the conserved C-terminus of assembly factor Krr1 as crucial for efficient release of the essential snR30. Furthermore, we show that snR30 is bound to the central domain of the 18S rRNA within a complex that contains assembly factors and ribosomal proteins. Our study shows how independent 18S domain maturation is guided by snR30 in coupling rRNA chaperoning with the delivery of ribosomal proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-22
AnnouncementXML	Submission_2025-05-22_03:02:29.480.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054031
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paulina Fischer
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-18 02:33:28	ID requested
⏵ 1	2025-05-22 03:02:29	announced

10.6019/PXD054031;

10.1038/S41467-025-59656-8;

submitter keyword: Yeast, snoRNP, ribosome biogenesis

Ed Hurt
contact affiliation	Biochemistry Center Heidelberg, University of Heidelberg, Germany
contact email	ed.hurt@bzh.uni-heidelberg.de
lab head
Paulina Fischer
contact affiliation	Biochemistry Center Heidelberg (BZH)
contact email	paulina.fischer@bzh.uni-heidelberg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054031

PRIDE project URI

• PRIDE
  1. PXD054031
     1. Label: PRIDE project
     2. Name: H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation – Dataset 1