PXD053985 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of rhLF expressed in Komagataella phaffii |
Description | Prior to the introduction of novel food ingredients into the food supply, safety risk assessments are required, and numerous prediction models have been developed and validated to evaluate safety. The allergenic risk potential of Helaina recombinant human lactoferrin (rhLF, Efferaâ„¢), produced in Komagataella phaffii (K. phaffii) was assessed by literature search, bioinformatics sequence comparisons to known allergens, glycan allergenicity assessment, and a simulated pepsin digestion model. The literature search identified no allergenic risk for Helaina rhLF, K. phaffii, or its glycans. Bioinformatics search strategies showed no significant risk for cross-reactivity or allergenicity between rhLF or the 36 residual host proteins and known human allergens. Helaina rhLF was also rapidly digested in simulated gastric fluid and its digestibility profile was comparable to human milk lactoferrin (hmLF), further demonstrating a low allergenic risk and similarity to the hmLF form. Collectively, these results demonstrate a low allergenic risk potential of Helaina rhLF and do not support the need for further clinical testing or serum IgE binding to evaluate Helaina rhLF for risk of food allergy prior to addition into the food supply. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_02:50:50.577.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD053985 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Philip Johnson |
SpeciesList | scientific name: Komagataella phaffii; NCBI TaxID: 460519; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-16 09:35:57 | ID requested | |
⏵ 1 | 2024-10-17 02:50:51 | announced | |
Publication List
10.3389/fimmu.2024.1380028; |
10.6019/PXD053985; |
Anaya Y, Rosario Martinez R, Goodman RE, Johnson P, Vajpeyi S, Lu X, Peterson R, Weyers SM, Breen B, Newsham K, Scottoline B, Clark AJ, Malinczak CA, . Front Immunol, 15():1380028(2024) [pubmed] |
Keyword List
submitter keyword: lactoferrin,allerg* |
Contact List
Philip Johnson |
contact affiliation | Department of Food Science and Technology, University of Nebraska, Lincoln |
contact email | philip.johnson@unl.edu |
lab head | |
Philip Johnson |
contact affiliation | University of Nebraska, Lincoln |
contact email | philip.johnson@unl.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053985
- Label: PRIDE project
- Name: Proteomic analysis of rhLF expressed in Komagataella phaffii