PXD053985

PXD053985 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of rhLF expressed in Komagataella phaffii
Description	Prior to the introduction of novel food ingredients into the food supply, safety risk assessments are required, and numerous prediction models have been developed and validated to evaluate safety. The allergenic risk potential of Helaina recombinant human lactoferrin (rhLF, Effera™), produced in Komagataella phaffii (K. phaffii) was assessed by literature search, bioinformatics sequence comparisons to known allergens, glycan allergenicity assessment, and a simulated pepsin digestion model. The literature search identified no allergenic risk for Helaina rhLF, K. phaffii, or its glycans. Bioinformatics search strategies showed no significant risk for cross-reactivity or allergenicity between rhLF or the 36 residual host proteins and known human allergens. Helaina rhLF was also rapidly digested in simulated gastric fluid and its digestibility profile was comparable to human milk lactoferrin (hmLF), further demonstrating a low allergenic risk and similarity to the hmLF form. Collectively, these results demonstrate a low allergenic risk potential of Helaina rhLF and do not support the need for further clinical testing or serum IgE binding to evaluate Helaina rhLF for risk of food allergy prior to addition into the food supply.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_02:50:50.577.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053985
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Johnson
SpeciesList	scientific name: Komagataella phaffii; NCBI TaxID: 460519;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-16 09:35:57	ID requested
⏵ 1	2024-10-17 02:50:51	announced

10.3389/fimmu.2024.1380028;

10.6019/PXD053985;

Anaya Y, Rosario Martinez R, Goodman RE, Johnson P, Vajpeyi S, Lu X, Peterson R, Weyers SM, Breen B, Newsham K, Scottoline B, Clark AJ, Malinczak CA, . Front Immunol, 15():1380028(2024) [pubmed]

submitter keyword: lactoferrin,allerg*

Philip Johnson
contact affiliation	Department of Food Science and Technology, University of Nebraska, Lincoln
contact email	philip.johnson@unl.edu
lab head
Philip Johnson
contact affiliation	University of Nebraska, Lincoln
contact email	philip.johnson@unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053985
     1. Label: PRIDE project
     2. Name: Proteomic analysis of rhLF expressed in Komagataella phaffii