PXD053973

PXD053973 is an original dataset announced via ProteomeXchange.

Title	HIIT and Pregnancy: Proteomic Insights into Metabolic and Immune Adaptations
Description	Physical activity is essential for maternal health during pregnancy, with WHO guidelines recommending 150 minutes of moderate-intensity aerobic exercise per week. Despite the established benefits, only 35% of pregnant women meet these guidelines, often due to fatigue, lack of time, and pregnancy discomfort. High-Intensity Interval Training (HIIT), characterized by short bursts of intense exercise followed by recovery periods, offers a time-efficient alternative that has been shown to be safe and enjoyable for both mother and fetus. This study aims to analyze the changes induced by a HIIT program in the urine proteome of pregnant women using the SWATH-MS technique, providing insights into molecular processes influenced by exercise.We observed significant alterations in the urinary proteome after a single HIIT session, with a higher scale of differentially expressed proteins compared to after 8 weeks of regular HIIT training. Notable changes included proteins involved in calcium ion binding, enzyme inhibitory activity, and immune system pathways, particularly neutrophil degranulation. Proteins such as ARSA, NPC2, MME, and CD177 showed significant regulation, indicating metabolic adaptations and immune responses. The observed downregulation of proteins like TFF2, associated with insulin resistance, suggests potential benefits of HIIT in preventing gestational diabetes.Our findings demonstrate that while a single session of HIIT induces immediate metabolic and immune changes, regular HIIT leads to adaptations, reflected in a decreased number of altered proteins over time. This research provides a novel proteomic perspective on the impact of HIIT during pregnancy, underscoring its potential to improve maternal health through targeted metabolic and immune system adaptations. Further studies are needed to confirm these findings and explore the long-term benefits of HIIT for pregnant women.
HostingRepository	PRIDE
AnnounceDate	2025-11-17
AnnouncementXML	Submission_2025-11-16_19:29:46.896.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominika Goik
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-16 02:34:16	ID requested
⏵ 1	2025-11-16 19:29:49	announced

10.1038/s41598-025-22991-3;

Goik D, Szumilewicz A, Musia, ł N, Santos-Rocha R, Wo, ł, yniec W, May LE, Czaplewska P, Proteomic changes induced by acute exercise and high-intensity interval training during pregnancy: a pilot randomised controlled trial. Sci Rep, 15(1):39317(2025) [pubmed]

submitter keyword: Physical activity, Urine samples, Pregnancy, Mass spectrometry,HIIT

dr. hab. Paulina Czaplewska, prof. UG
contact affiliation	Mass Spectroscopy Laboratory Intercollegiate Faculty of Biotechnology UG&MUG
contact email	paulina.czaplewska@ug.edu.pl
lab head
Dominika Goik
contact affiliation	University of Gdańsk
contact email	dominika.goik98@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD053973

PRIDE project URI

• PRIDE
  1. PXD053973
     1. Label: PRIDE project
     2. Name: HIIT and Pregnancy: Proteomic Insights into Metabolic and Immune Adaptations