PXD053951

PXD053951 is an original dataset announced via ProteomeXchange.

Title	IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells
Description	Interferon-induced transmembrane proteins (IFITMs) are frequently overexpressed in cancer cells, including cervical carcinoma cells, and play a role in the progression of various cancer types. However, their mechanisms of action remain incompletely understood. In the present study, by employing a combination of surface membrane protein isolation and quantitative mass spectrometry, it was comprehensively described how the IFITM1 protein influences the composition of the cervical cancer cell surfaceome. Additionally, the effects of interferon-γ on protein expression and cell surface exposure were evaluated in the presence and absence of IFITM1. The IFITM1-regulated membrane and membrane-associated proteins identified are involved mainly in processes such as endocytosis and lysosomal transport, cell-cell and cell-extracellular matrix adhesion, antigen presentation and the immune response. To complement the proteomic data, gene expression was analyzed using reverse transcription-quantitative PCR to distinguish whether the observed changes in protein levels were attributable to transcriptional regulation or differential protein dynamics. Furthermore, the proteomic and gene expression data are supported by functional studies demonstrating the impact of the IFITM1 and IFITM3 proteins on the adhesive, migratory and invasive capabilities of cervical cancer cells, as well as their interactions with immune cells.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:12:48.660.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lenka Hernychova
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-15 06:55:17	ID requested
⏵ 1	2025-05-25 16:12:49	announced

Friedlov, á N, Bortl, í, kov, á L, Dosed, ě, lov, á L, Uhr, í, k L, Hupp TR, Hernychov, á L, Vojt, ě, š, ek B, Nekulov, á M, IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells. Oncol Rep, 53(6):(2025) [pubmed]

10.3892/or.2025.8904;

submitter keyword: Human, cervicospheres, Interferon inducible transmembrane protein, mass spectrometry, ALCAM, SILAC, migration, invasion

Marta Nekulova
contact affiliation	RECAMO, Masaryk Memorial Cancer Institute, Zluty kopec 7,Brno, 656 53, CZ
contact email	M.Nekulova@seznam.cz
lab head
Lenka Hernychova
contact affiliation	Masaryk Memorial Cancer Institute
contact email	avochynreh@seznam.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053951

PRIDE project URI

• PRIDE
  1. PXD053951
     1. Label: PRIDE project
     2. Name: IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells