⮝ Full datasets listing
PXD053949
PXD053949 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Species-Specific Epigenetic Signature Associate with Heat Stress Tolerance in a Perennial Tree Species Populus |
| Description | Epigenetic regulation in annual plants is recognized as a key component of recurring stress adaptation, but reports on perennial tree species are limited. In this study, two contrasting tree species, Populus trichocarpa and Populus deltoides, and a F1 hybrid cross between them showed species-specific epigenetic and physiological responses to heat stress following priming. By analyzing whole-genome methylation, transcriptomics, proteomics, metabolomics, and photosynthesis parameters, we found that P. deltoides has adapted epigenetically to heat stress, resulting in improved photosynthetic efficiency compared to P. trichocarpa. Conversely, P. trichocarpa displayed stress signaling and defense mechanisms that could not sustain a net assimilation rate despite maintaining higher gas exchange. Heat stress following priming in hybrid plants increased transcript levels of thermotolerance-related transcription factors, such as SPL12. Selected regions in the promoter of SPL12 showed differential methylation between direct heat stress and priming followed by heat stress. This, in turn, resulted in the upregulation of downstream genes and associated increases in protein and metabolite abundance for stress adaptation. Consequently, hybrid plants showed enhanced photosynthesis and gas exchange rates, a trait lacking in P. trichocarpa. These results imply that priming may not be universally effective in enhancing plant performance under stress, particularly in perennial tree species. However, priming can acclimate the perennial tree species P. deltoides to withstand elevated temperature stress better. Our study has demonstrated that priming-based stress adaptation are species-specific but can be attained through crossbreeding, indicating its potential use in breeding programs. |
| HostingRepository | MassIVE |
| AnnounceDate | 2026-01-28 |
| AnnouncementXML | Submission_2026-01-28_10:35:55.039.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Dana Carper |
| SpeciesList | scientific name: Populus; common name: poplars; NCBI TaxID: 3689; scientific name: Populus trichocarpa; common name: black cottonwood; NCBI TaxID: 3694; scientific name: Populus deltoides; NCBI TaxID: 3696; scientific name: Populus trichocarpa x Populus deltoides; NCBI TaxID: 3695; |
| ModificationList | Carbamidomethyl; Oxidation |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2024-07-15 05:06:52 | ID requested | |
| ⏵ 1 | 2026-01-28 10:35:55 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Epigenetics, DNA methylation, Heat stress |
Contact List
| Paul Abraham | |
|---|---|
| contact affiliation | ORNL |
| contact email | pav@ornl.gov |
| lab head | |
| Dana Carper | |
| contact affiliation | Oak Ridge National Laboratory |
| contact email | carperdl@ornl.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive-ftp.ucsd.edu/v08/MSV000095335/ |
