PXD053937

PXD053937 is an original dataset announced via ProteomeXchange.

Title	Analysis of signaling networks in response to radioimmunotherapy in T-cell lymphoma mouse model
Description	Cutaneous T-cell lymphomas (CTCLs) constitute a rare and heterogeneous group of lymphoid malignant neoplasms that initially involve the skin and are considered neglected clinical entities with very limited therapeutic options. Lymphoma cells (both B and T-cells) are extremely radiosensitive compared to solid adenocarcinomas. Nevertheless, many CTCL patients progress to an advanced stage of the disease, in which the malignant cells disseminate through the body, greatly limiting the use of conventional external beam radiotherapy. In the last decade, CD30 emerged as a promising target with high expression on malignant B and T-cells and limited expression in healthy tissues. Thus, recently we have developed radioimmunotherapy (RIT) with anti-CD30 mAbs coupled to radionuclides to selectively deliver high doses of radiation to T-cell lymphomas while preventing radiotoxicity in healthy tissues. Importantly, we have employed novel therapeutic radionuclide Terbium-161, which emits both β--radiation and Auger and conversion electrons with high linear energy transfer and an ultra-short range in tissue. Currently, it is important to understand how this type of RIT with Tb-161 alters signaling networks and protein levels. Thus, the goal of this project is to analyze cellular response to Tb-161 radioimmunotherapy by proteomics and phosphoproteomics in our T-cell lymphoma mouse model. We anticipate that our study will not only broaden our understanding of cancer radiobiology but also identify potential RIT-induced and survival-driving mechanisms and pathways, which targeting can be further explored for the development of novel radio-sensitizing strategies.
HostingRepository	PRIDE
AnnounceDate	2025-06-04
AnnouncementXML	Submission_2025-06-04_02:07:38.086.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michal Grzmil
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-14 13:34:38	ID requested
⏵ 1	2025-06-04 02:07:38	announced

10.2967/JNUMED.124.268805;

submitter keyword: lymphoma, terbium-161,TMT, phosphoproteomics, radioimmunotherapy

Michal Grzmil
contact affiliation	Paul Scherrer Institute, Center for Radiopharmaceutica Sciences, 5232 Villigen PSI, Switzerland
contact email	michal.grzmil@psi.ch
lab head
Michal Grzmil
contact affiliation	Paul Scherrer Institute Center for Radiopharmaceutical Sciences
contact email	michal.grzmil@psi.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/06/PXD053937

PRIDE project URI

• PRIDE
  1. PXD053937
     1. Label: PRIDE project
     2. Name: Analysis of signaling networks in response to radioimmunotherapy in T-cell lymphoma mouse model