PXD053932

PXD053932 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Immunoprecipitation of Toxoplasma gondii mETC supercomplex mutant
Description	The apicomplexan parasite Toxoplasma gondii has a divergent mitochondrial electron transport chain (mETC). Complex III and IV associate together to form supercomplexes. We identified and deleted a Complex IV subunit (ApiCox10) which mediates this interaction. We perfomred immunoprecipitation of complex IV, via an HA epitope tagged Cox2a subunit in a parental and ApiCox10 knockout lines extracted in the mild detergent digitonin and analysed the elution via LFQ mass spectrometry. In the parental line, by immunoprecipitating Complex IV, complex III is also recovered, whereas in the ApiCox10 mutant only Complex IV is recovered due to impaired supercomplex formation.
HostingRepository	PRIDE
AnnounceDate	2025-01-15
AnnouncementXML	Submission_2025-01-15_07:41:08.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andree Maclean
SpeciesList	scientific name: Toxoplasma gondii; NCBI TaxID: 5811; scientific name: Toxoplasma gondii; NCBI TaxID: NCBITaxon:5811;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-13 10:27:11	ID requested
⏵ 1	2025-01-15 07:41:08	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: Toxoplasma, complex III, mETC, supercomplex, mitochondria, complex IV

submitter keyword: Toxoplasma, complex III, mETC, supercomplex, mitochondria, complex IV

Contact List

Lilach Sheiner
contact affiliation	School of Infection and Immunity, MVLS, University of Glasgow
contact email	lilach.sheiner@glasgow.ac.uk
lab head
Andree Maclean
contact affiliation	University of Glasgow
contact email	andrew.maclean@glasgow.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD053932
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD053932

PRIDE project URI

Repository Record List

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