PXD053927

PXD053927 is an original dataset announced via ProteomeXchange.

Title	Glutathione Oxidation in Cerebrospinal Fluid as a Biomarker of Oxidative Stress in Amyotrophic Lateral Sclerosis [Oxiproteomics]
Description	We performed unbiased quantitative mass spectrometry (MS)-based analysis to measure changes in protein abundance and oxidation in cerebrospinal fluid (CSF) from a cohort of Amyotrophic Lateral Sclerosis (ALS) patients and healthy controls at two time points (approximately four months apart) to capture disease progression. In addition, we developed a sensitive and targeted quantitative MS method to measure glutathione oxidation state in the same sets of CSF samples.
HostingRepository	PRIDE
AnnounceDate	2025-07-14
AnnouncementXML	Submission_2025-07-13_16:20:12.507.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Trong Khoa Pham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-ethylmaleimide derivatized cysteine; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-12 22:03:35	ID requested
⏵ 1	2025-07-13 16:20:13	announced

10.1186/s40035-025-00496-3;

Pham TK, Verber N, Turner MR, Malaspina A, Collins MO, Mead RJ, Shaw PJ, Glutathione oxidation in cerebrospinal fluid as a biomarker of oxidative stress in amyotrophic lateral sclerosis. Transl Neurodegener, 14(1):36(2025) [pubmed]

submitter keyword: glutathione,ALS, CSF, Oxiproteomics, cysteine oxidation, biomarkers

Trong Khoa Pham
contact affiliation	School of Biosciences The University of Sheffield Western Bank Sheffield S10 2TN
contact email	khoa.pham@sheffield.ac.uk
lab head
Trong Khoa Pham
contact affiliation	Department of Chemical and Biological Engineering
contact email	khoa.pham@sheffield.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053927
     1. Label: PRIDE project
     2. Name: Glutathione Oxidation in Cerebrospinal Fluid as a Biomarker of Oxidative Stress in Amyotrophic Lateral Sclerosis [Oxiproteomics]