PXD053925

PXD053925 is an original dataset announced via ProteomeXchange.

Title	A functional connection between the Microprocessor and a variant NEXT complex
Description	In mammalian cells, primary miRNAs are cleaved at their hairpin structures by the Microprocessor complex, whose core is composed of DROSHA and DGCR8. Here, we show that 5’flanking regions, resulting from Microprocessor cleavage, are targeted by the RNA exosome in mouse embryonic stem cells (mESCs). This is facilitated by a physical link between DGCR8 and the nuclear exosome targeting (NEXT) component ZCCHC8. Surprisingly, however, both biochemical and mutagenesis studies demonstrate that a variant NEXT complex, containing the RNA helicase MTR4 but devoid of the RNA-binding protein RBM7, is the active entity. This Microprocessor-NEXT variant also targets stem-loop containing RNAs expressed from other genomic regions, such as enhancers. In contrast, Microprocessor does not contribute to the turnover of less structured NEXT substrates. Our results therefore demonstrate that MTR4-ZCCHC8 can link to either RBM7 or DGCR8/DROSHA to target different RNA substrates depending on their structural context.
HostingRepository	PRIDE
AnnounceDate	2026-05-23
AnnouncementXML	Submission_2026-05-22_16:45:23.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Andersen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-12 14:16:27	ID requested
⏵ 1	2026-05-22 16:45:27	announced

10.1016/j.molcel.2024.10.015;

Imamura K, Garland W, Schmid M, Jakobsen L, Sato K, Rouvi, è, re JO, Jakobsen KP, Burlacu E, Lopez ML, Lykke-Andersen S, Andersen JS, Jensen TH, A functional connection between the Microprocessor and a variant NEXT complex. Mol Cell, 84(21):4158-4174.e6(2024) [pubmed]

submitter keyword: ZCCHC8-DGCR8 interaction, RNA structure,Microprocessor, nuclear RNA decay, the NEXT complex

Jens S. Andersen
contact affiliation	University of Southern Denmark, Department of Biochemistry and Molecular Biology, Campusvej 55, 5230 Odense M, Denmark
contact email	jens.andersen@bmb.sdu.dk
lab head
Jens Andersen
contact affiliation	University of Southern Denmark, Department og Biochemistry and Molecular Biology
contact email	jens.andersen@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/05/PXD053925

PRIDE project URI

• PRIDE
  1. PXD053925
     1. Label: PRIDE project
     2. Name: A functional connection between the Microprocessor and a variant NEXT complex