PXD053912

PXD053912 is an original dataset announced via ProteomeXchange.

Title	Erosive tooth wear and its association to risk, saliva and biofilm composition in Colombian adolescents
Description	The aim of this study was to compare the protein composition of the acquired pellicle from adolescents with Erosive Tooth Wear (ETW) caries and sound. Calibrated examiners in BEWE Index and ICDAS-merged Epi criteria assessed ETW and caries in a sample of 454 systemically healthy adolescents aged 12 to 15 years old. From them, 30 subjects were selected for this study, presenting either Sound enamel (n=10, Total BEWE sum <8 and without extensive/cavitated caries lesions), ETW (n=10, Total BEWE sum ≥9 and without extensive/cavitated caries lesions) or Caries (n=10, Total BEWE sum <8 and with at least one Extensive caries lesion). Two hours formation acquired pellicle samples were taken from tooth surfaces (Buccal/Occlusal/Palatal/Lingual). Protein composition was analysed by Liquid Chromatography Tandem Mass Spectrometry. Using mean reporter ion values relative abundances of proteins were compared among the three groups to calculate for fold changes. Protein increases or decreases of 2-fold were reported (t-test, p<0.05). Gene Ontology (GO) of the included proteins was assigned. In the results of this project were found a total of 127 proteins in the acquired pellicle. The GO analyses showed that the majority of detected proteins were stress-response related. The ETW group disclosed up-regulated relative abundance of Antileucoprotease; Histatin and Prolactin-induced protein Hemoglobin subunits Alpha (HBA) and Beta (HBB) showed decreased relative abundances in the ETW and Caries groups when compared to the Sound group. As a conclusion, the acquired pellicle from individuals with ETW showed differences when compared to other dental conditions, with an increase in relative abundance of some stress response associated proteins in ETW and a decrease in proteins related to salivary protection against acid challenges.
HostingRepository	PRIDE
AnnounceDate	2026-01-16
AnnouncementXML	Submission_2026-01-16_02:46:17.794.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Lynham
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-12 04:54:49	ID requested
⏵ 1	2026-01-16 02:46:18	announced

10.1159/000541026;

submitter keyword: dental pellicle, tandem mass spectrometry,tooth wear, salivary proteins

Prof. Stefania Martignon
contact affiliation	UNICA Caries Research Unit - Research Department Universidad El Bosque Bogota, Colombia
contact email	martignonstefania@unbosque.edu.co
lab head
Steven Lynham
contact affiliation	King's College London
contact email	steven.lynham@kcl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053912
     1. Label: PRIDE project
     2. Name: Erosive tooth wear and its association to risk, saliva and biofilm composition in Colombian adolescents