PXD053911

PXD053911 is an original dataset announced via ProteomeXchange.

Title	CellEKT: A chemical proteomics assay to profile cellular target engagement of kinase inhibitors
Description	Activity-based protein profiling is a powerful tool to study target engagement in complex biological environments. XO44, ALX005 and ALX011 are chemical probes based on the scaffold of a promiscuous kinase binder, which covalently reacts with a conserved lysine in the ATP-binding pocket of kinases. Pretreatment of cells with a drug (candidate) results in competition of XO44/ALX005/ALX011 binding, which can be interpreted as in situ target engagement. Here CellEKT is presented, a workflow which is optimized for kinome coverage, reproducibility and data processing. This dataset pertains to the selectivity testing of Dasatinib in MV4-11 cells using a probe cocktail of XO44, ALX005 and ALX011.
HostingRepository	PRIDE
AnnounceDate	2025-04-08
AnnouncementXML	Submission_2025-04-08_07:14:52.860.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joel Rüegger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-12 04:03:37	ID requested
⏵ 1	2025-04-08 07:14:53	announced

10.1016/J.MCPRO.2025.100961;

submitter keyword: ABPP, drug discovery, ALX005,CellEKT, chemical proteomics, ALX011, kinase, XO44

Mario van der Stelt
contact affiliation	Professor of Molecular Physiology, Leiden Institute of Chemistry, Leiden University, The Netherlands
contact email	m.van.der.stelt@chem.leidenuniv.nl
lab head
Joel Rüegger
contact affiliation	Leiden University
contact email	j.ruegger@lic.leidenuniv.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053911

PRIDE project URI

• PRIDE
  1. PXD053911
     1. Label: PRIDE project
     2. Name: CellEKT: A chemical proteomics assay to profile cellular target engagement of kinase inhibitors