PXD053909

PXD053909 is an original dataset announced via ProteomeXchange.

Title	E. coli expressed SECRET AGENT O-GlcNAc modifies threonine 829 of GIGANTEA
Description	The Arabidopsis thaliana glycosyl transferases SPINDLY (SPY) and SECRET AGENT (SEC) modify nuclear and cytosolic proteins with O-linked fucose or O-linked Nacetylglucosamine (O-GlcNAc), respectively. O-fucose and O-GlcNAc modifications can occur at the same sites. SPY interacts physically and genetically with GIGANTEA (GI), suggesting that it could be modified by both enzymes. Previously, we found that, when co-expressed in Escherichia coli, SEC modifies GI; however, the modification site was not determined. By analyzing the overlapping sub-fragments of GI, we identified a region that was modified by SEC in E. coli. Modification was undetectable when threonine 829 (T829) was mutated to alanine, while the T834A and T837A mutations reduced the modification, suggesting that T829 was the primary or the only modification site. Mapping using mass spectrometry detected only the modification of T829. Previous studies have shown that the positions modified by SEC in E. coli are modified in planta, suggesting that T829 is O-GlcNAc modified in planta.
HostingRepository	PRIDE
AnnounceDate	2024-07-15
AnnouncementXML	Submission_2024-07-15_11:17:13.598.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053909
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Neil Olszewski
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	O-(N-acetylamino)glucosyl-L-threonine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-12 02:34:48	ID requested
⏵ 1	2024-07-15 11:17:14	announced

10.6019/PXD053909;

submitter keyword: Arabidopsis thaliana, GIGANTEA, protein modification, O-GlcNAc,E. coli

Neil Olszewski
contact affiliation	Department of Plant & Microbial Biology, University of Minnesota,St. Paul, MN, USA
contact email	neil@umn.edu
lab head
Neil Olszewski
contact affiliation	University of Minnestoa Department of Plant & Microbial Biology 140 Gortner Laboratory 1479 Gortner Avenue St. Paul, MN 55108
contact email	neil@umn.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053909
     1. Label: PRIDE project
     2. Name: E. coli expressed SECRET AGENT O-GlcNAc modifies threonine 829 of GIGANTEA