PXD053902 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrated proteomic and global phosphoproteomic analysis of cisplatin-induced apoptosis in A549 cells |
Description | Protein phosphorylation, a widely occurring and significant post-translational modification, is integral to various biological processes. We previously utilized a protein affinity probe to identify genes damaged by cisplatin, revealing that it inflicts substantial damage on protein kinase and protein phosphatase genes. In this study, we investigated cisplatin-induced alterations in the proteome and global phosphoproteome of A549 cells. Employing Fe-IMAC beads and tyrosine phosphorylation enrichment antibodies, we identified 6944 protein groups and 18,274 phosphorylation sites on 4,915 proteins across three biological replicates of both cisplatin-treated A549 cells and control cells. Among these, 730 tyrosine phosphorylation sites were identified—marking the most substantial discovery of such sites in A549 cells following cisplatin treatment. Bioinformatics analysis indicated that the proteins exhibiting significant phosphorylation level changes, which are predominantly involved in RNA processing, modification, transcription, translation, and the spliceosome. This suggests that cisplatin-induced damage to protein kinases and phosphatases may disrupt the normal function of these proteins, consequently impairing DNA replication, RNA translation, and shearing, ultimately culminating in tumor cell death. Moreover, we cross-referenced our proteomic data with our previously obtained cisplatin-damaged genes, observing that the majority of down-regulated proteins derived from cisplatin-induced gene damage. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-20 |
AnnouncementXML | Submission_2024-10-20_05:47:29.747.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | luyu qi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-11 21:07:17 | ID requested | |
⏵ 1 | 2024-10-20 05:47:30 | announced | |
Publication List
Keyword List
submitter keyword: phosphoproteomic, cisplatin-damage genes,proteomic, cisplatin, A549 cells |
Contact List
Jianzhong Shao |
contact affiliation | College of Life Sciences, Key Laboratory for Cell and Gene Engineering of Zhejiang Province, Zhejiang University, Hangzhou, China |
contact email | shaojz@zju.edu.cn |
lab head | |
luyu qi |
contact affiliation | Zhejiang University |
contact email | luyuqi1993@foxmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053902
- Label: PRIDE project
- Name: Integrated proteomic and global phosphoproteomic analysis of cisplatin-induced apoptosis in A549 cells