PXD053872

PXD053872 is an original dataset announced via ProteomeXchange.

Title	Co-culture CRISPR screens reveal ATG9A as a regulator to macrophage-mediated cytotoxicity in cancer
Description	This study investigates the role of cancer cell surface proteins in modulating the response of cancer cells to macrophage-induced cytotoxicity. Utilizing two targeted CRISPR screens using OVCAR-8 cell line and a targeted CRISPR library for cancer cell surface proteins, ATG9A was identified as a key regulator. ATG9A KO increased the sensitivity of cancer cells to macrophage-induced cytotoxicity. Subsequent surface and whole-cell mass spectrometry analysis elucidated the underlying mechanisms; ATG9A KO in cancer cells not only enhances macrophage infiltration and activation but also plays a crucial role in the plasma membrane repair following macrophage co-culture. These findings reveal novel targets for enhancing macrophage-mediated cytotoxicity in cancer, offering additional avenues for therapeutic intervention.
HostingRepository	PRIDE
AnnounceDate	2025-04-29
AnnouncementXML	Submission_2025-04-28_20:41:29.793.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Abhilash Barpanda
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-11 00:43:04	ID requested
⏵ 1	2025-04-28 20:41:30	announced

Dataset with its publication pending

submitter keyword: ATG9A

Macrophage-mediated cytotoxicity

CRISPR screens

Cancer cell surface proteins

Arun Wiita
contact affiliation	Associate Professor, Department of Laboratory Medicine, UCSF
contact email	Arun.Wiita@ucsf.edu
lab head
Abhilash Barpanda
contact affiliation	IIT Bombay
contact email	avilash94@iitb.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053872
     1. Label: PRIDE project
     2. Name: Co-culture CRISPR screens reveal ATG9A as a regulator to macrophage-mediated cytotoxicity in cancer