PXD053872 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Co-culture CRISPR screens reveal ATG9A as a regulator to macrophage-mediated cytotoxicity in cancer |
Description | This study investigates the role of cancer cell surface proteins in modulating the response of cancer cells to macrophage-induced cytotoxicity. Utilizing two targeted CRISPR screens using OVCAR-8 cell line and a targeted CRISPR library for cancer cell surface proteins, ATG9A was identified as a key regulator. ATG9A KO increased the sensitivity of cancer cells to macrophage-induced cytotoxicity. Subsequent surface and whole-cell mass spectrometry analysis elucidated the underlying mechanisms; ATG9A KO in cancer cells not only enhances macrophage infiltration and activation but also plays a crucial role in the plasma membrane repair following macrophage co-culture. These findings reveal novel targets for enhancing macrophage-mediated cytotoxicity in cancer, offering additional avenues for therapeutic intervention. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-29 |
AnnouncementXML | Submission_2025-04-28_20:41:29.793.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Abhilash Barpanda |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | iodoacetamide derivatized residue |
Instrument | timsTOF Pro 2 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-11 00:43:04 | ID requested | |
⏵ 1 | 2025-04-28 20:41:30 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: ATG9A |
Macrophage-mediated cytotoxicity |
CRISPR screens |
Cancer cell surface proteins |
Contact List
Arun Wiita |
contact affiliation | Associate Professor, Department of Laboratory Medicine, UCSF |
contact email | Arun.Wiita@ucsf.edu |
lab head | |
Abhilash Barpanda |
contact affiliation | IIT Bombay |
contact email | avilash94@iitb.ac.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053872
- Label: PRIDE project
- Name: Co-culture CRISPR screens reveal ATG9A as a regulator to macrophage-mediated cytotoxicity in cancer