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PXD053872

PXD053872 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCo-culture CRISPR screens reveal ATG9A as a regulator to macrophage-mediated cytotoxicity in cancer
DescriptionThis study investigates the role of cancer cell surface proteins in modulating the response of cancer cells to macrophage-induced cytotoxicity. Utilizing two targeted CRISPR screens using OVCAR-8 cell line and a targeted CRISPR library for cancer cell surface proteins, ATG9A was identified as a key regulator. ATG9A KO increased the sensitivity of cancer cells to macrophage-induced cytotoxicity. Subsequent surface and whole-cell mass spectrometry analysis elucidated the underlying mechanisms; ATG9A KO in cancer cells not only enhances macrophage infiltration and activation but also plays a crucial role in the plasma membrane repair following macrophage co-culture. These findings reveal novel targets for enhancing macrophage-mediated cytotoxicity in cancer, offering additional avenues for therapeutic intervention.
HostingRepositoryPRIDE
AnnounceDate2025-04-29
AnnouncementXMLSubmission_2025-04-28_20:41:29.793.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAbhilash Barpanda
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumenttimsTOF Pro 2
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-11 00:43:04ID requested
12025-04-28 20:41:30announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: ATG9A
Macrophage-mediated cytotoxicity
CRISPR screens
Cancer cell surface proteins
Contact List
Arun Wiita
contact affiliationAssociate Professor, Department of Laboratory Medicine, UCSF
contact emailArun.Wiita@ucsf.edu
lab head
Abhilash Barpanda
contact affiliationIIT Bombay
contact emailavilash94@iitb.ac.in
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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