PXD053858

PXD053858 is an original dataset announced via ProteomeXchange.

Title	Influenza A virus-induced production of PI4P at the endoplasmic reticulum involves ATG16L1 and promotes the egress of viral ribonucleoproteins
Description	To further understand the molecular mechanisms by which RAB11A is hijacked in IAV-infected cells, we sought to identify viral-induced changes in RAB11A interactome. To this end, we used a TurboID-based proximity labelling approach, most suitable for the detection of weak or transient protein-protein interactions (Figure 2A). TurboID is a highly active biotin ligase that rapidly converts biotin into biotin–AMP, a reactive intermediate which covalently labels proximal proteins within a 10-30 nm radius(REF DOI : 10.1038/s41596-020-0399-0). The RAB11A protein was fused to TurboID and stably expressed in A549 cells by lentiviral transduction (Supplementary Figure 2A western blot). The resulting A549-TurboID-RAB11A cell population was infected with the WSN virus at a MOI of 5 PFU/cell - conditions in which all cells are positive for the viral nucleoprotein (NP) upon immunostaining at 8 hpi (Supplementary Figure 2B IF-NP), or mock-infected. At 8 hpi, biotin was added to the culture medium for 10 min before cell lysates were prepared, and biotinylated proteins were purified with streptavidin beads, as described in REF. A fraction of the total lysates and the biotinylated eluates were analysed by mass spectrometry based-proteomics. Four independent biological replicates were performed.
HostingRepository	PRIDE
AnnounceDate	2025-06-19
AnnouncementXML	Submission_2025-06-19_06:06:34.126.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-10 12:36:03	ID requested
⏵ 1	2025-06-19 06:06:34	announced

Dataset with its publication pending

submitter keyword: Human, shotgun proteomics, TurboID, influenza A virus

Nadia Naffakh
contact affiliation	Institut Pasteur, Université Paris Cité, CNRS UMR3569, Unité Biologie des ARN et Virus Influenza, Paris, France
contact email	nadia.naffakh@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053858
     1. Label: PRIDE project
     2. Name: Influenza A virus-induced production of PI4P at the endoplasmic reticulum involves ATG16L1 and promotes the egress of viral ribonucleoproteins