PXD053856

PXD053856 is an original dataset announced via ProteomeXchange.

Title	Astrocytic oxytocin receptors mediate behavioral stress response through Gαi signaling in the central amygdala
Description	Anticipated reactions to stressful situations are vital for the survival and well-being of organisms, and abnormal reactions are involved in stress-related disorders. The neuropeptide oxytocin is a key modulator ensuring well-adapted stress responses. Oxytocin acts on both neurons and astrocytes, but the molecular and cellular mechanisms mediating stress response remain poorly understood. Here, we focus on the amygdala, a crucial hub that integrates and processes sensory information through oxytocin-dependent mechanisms. Using an acute stress paradigm in mice, genetic and pharmacological manipulations combined with molecular, morphological, electrophysiological and behavioral approaches, we reveal that oxytocinergic modulation of the freezing response to stress is mediated by transient, Gαi-dependent retraction of astrocytic processes, followed by enhanced neuronal sensitivity to extracellular potassium in the amygdala. Our findings elucidate a pivotal role for oxytocin-dependent signaling in astrocytes that is required for proper anticipated behavioral response to stressful situations.
HostingRepository	PRIDE
AnnounceDate	2026-09-02
AnnouncementXML	Submission_2026-09-02_03:57:29.654.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Syed Azmal Ali
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-10 11:30:35	ID requested
1	2025-12-09 11:17:15	announced
2	2026-09-02 03:46:13	announced	2026-09-02: Updated project metadata.
⏵ 3	2026-09-02 03:57:30	announced	2026-09-02: Updated project metadata.

10.1038/s41467-025-68114-4;

Baudon A, Grelot V, Wang KY, Althammer F, Denis C, Rich, é, -Piotaix P, Ali AS, Yan Y, Castillo D, í, az F, Piacentini F, Clauss Creusot E, Hovhannisyan V, Schubert T, Kleinwaechter AC, Eisemann EM, Muhammad JA, Helen J, Lakomy T, Krabichler Q, Breton R, Andry V, Carrillo-de Sauvage MA, Demais V, C, è, s A, Franke F, Schommer F, Kremer M, Di Benedetto B, Goumon Y, Schaaf CP, Grinevich V, Rouach N, Darbon P, Neumann ID, Adamantidis A, Busnelli M, Benhaim L, Krijgsveld J, Pfrieger FW, Charlet A, i-dependent remodeling of astrocytes to shape neuronal response in the amygdala. Nat Commun, 17(1):1364(2025) [pubmed]

submitter keyword: Astrocytic

amygdala

Proteomics

Prof. Dr. Jeroen Krijgsveld
contact affiliation	German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
contact email	j.krijgsveld@dkfz-heidelberg.de
lab head
Syed Azmal Ali
contact affiliation	German Cancer Research Center
contact email	azmal786@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD053856

PRIDE project URI

• PRIDE
  1. PXD053856
     1. Label: PRIDE project
     2. Name: Astrocytic oxytocin receptors mediate behavioral stress response through Gαi signaling in the central amygdala