PXD053835

PXD053835 is an original dataset announced via ProteomeXchange.

Title	EphA2 regulates vascular permeability and prostate cancer metastasis via modulation of cell junction protein phosphorylation
Description	Prostate cancer morbidity and mortality obviate a need for more effective targeted therapies. One potential target is EphA2, although paradoxically, pro- and anti-oncogenic effects have been shown to be mediated by EphA2. We demonstrate that unique activating and blocking EphA2-targeting monoclonal antibodies display opposing tumor-suppressive and oncogenic properties in vivo. To further explore this complexity, we performed detailed phosphoproteomic analysis following ligand-induced EphA2 activation. Our analysis identified altered phosphorylation of 73 downstream proteins related to the PI3K/AKT/mTOR and ERK/MAPK pathways, with the majority implicated in cell junction and cytoskeletal organization, cell motility, and tumor metastasis. We demonstrate that the adaptor protein SHB is an essential component in mediating the inhibition of the Erk/MAPK pathway in response to EphA2 receptor activation. Furthermore, we identify the adherence junction protein afadin as an EphA2-regulated phosphoprotein which is involved in prostate cancer migration and invasion.
HostingRepository	PRIDE
AnnounceDate	2024-11-11
AnnouncementXML	Submission_2024-11-10_20:43:09.957.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carolin Offenhaeuser
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-10 00:14:44	ID requested
⏵ 1	2024-11-10 20:43:10	announced

10.1038/S41388-024-03206-X;

submitter keyword: Afadin, SHB,Phosphoproteomics, EphA2, SILAC, Eph receptor

Bryan Day
contact affiliation	Cancer Program, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia
contact email	Bryan.Day@qimrberghofer.edu.au
lab head
Carolin Offenhaeuser
contact affiliation	Cancer Program, QIMR Berghofer Medical Research Institute, Brisbane, QLD 4006, Australia
contact email	c.offenhaeuser@yahoo.com.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD053835

PRIDE project URI

• PRIDE
  1. PXD053835
     1. Label: PRIDE project
     2. Name: EphA2 regulates vascular permeability and prostate cancer metastasis via modulation of cell junction protein phosphorylation