PXD053797

PXD053797 is an original dataset announced via ProteomeXchange.

Title	RNA Encoded Peptide Barcodes Enable Efficient in vivo Screening of RNA Delivery Systems
Description	LNPs have been demonstrated to hold great promise for the clinical advancement of RNA therapeutics. Continued exploration of LNPs for application in new disease areas requires identification and optimisation of leads in a high throughput way. Currently available high throughput in vivo screening platforms are well suited to screen for cellular uptake but less so for functional cargo delivery. We report on a platform which measures functional delivery of LNPs using unique peptide ‘barcodes’. We describe the design and selection of the peptide barcodes and the evaluation of these for the screening of LNPs. We show that proteomic analysis of peptide barcodes correlates with quantification and efficacy of barcoded reporter proteins both in vitro and in vivo and, that the ranking of selected LNPs using peptide barcodes in a pool correlates with ranking using alternative methods in groups of animals treated with individual LNPs. We show that this system is sensitive, selective, and capable of reducing the size of an in vivo study by screening up to 10 unique formulations in a single pool, thus accelerating the discovery of new technologies for mRNA delivery.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_03:07:20.320.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nitin Rustogi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-09 06:52:31	ID requested
⏵ 1	2024-10-17 03:07:21	announced

10.1093/nar/gkae648;

Odunze U, Rustogi N, Devine P, Miller L, Pereira S, Vashist S, Snijder HJ, Corkill D, Sabirsh A, Douthwaite J, Bond N, Desai A, RNA encoded peptide barcodes enable efficient in vivo screening of RNA delivery systems. Nucleic Acids Res, 52(16):9384-9396(2024) [pubmed]

submitter keyword: LNP, proteomics,RNA, in vivo, high throughput, barcodes

Paul Devine
contact affiliation	Astrazeneca
contact email	paul.devine@astrazeneca.com
lab head
Nitin Rustogi
contact affiliation	Astrazeneca
contact email	nitin.rustogi@astrazeneca.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053797

PRIDE project URI

• PRIDE
  1. PXD053797
     1. Label: PRIDE project
     2. Name: RNA Encoded Peptide Barcodes Enable Efficient in vivo Screening of RNA Delivery Systems