PXD053797 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | RNA Encoded Peptide Barcodes Enable Efficient in vivo Screening of RNA Delivery Systems |
Description | LNPs have been demonstrated to hold great promise for the clinical advancement of RNA therapeutics. Continued exploration of LNPs for application in new disease areas requires identification and optimisation of leads in a high throughput way. Currently available high throughput in vivo screening platforms are well suited to screen for cellular uptake but less so for functional cargo delivery. We report on a platform which measures functional delivery of LNPs using unique peptide ‘barcodes’. We describe the design and selection of the peptide barcodes and the evaluation of these for the screening of LNPs. We show that proteomic analysis of peptide barcodes correlates with quantification and efficacy of barcoded reporter proteins both in vitro and in vivo and, that the ranking of selected LNPs using peptide barcodes in a pool correlates with ranking using alternative methods in groups of animals treated with individual LNPs. We show that this system is sensitive, selective, and capable of reducing the size of an in vivo study by screening up to 10 unique formulations in a single pool, thus accelerating the discovery of new technologies for mRNA delivery. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-17 |
AnnouncementXML | Submission_2024-10-17_03:07:20.320.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nitin Rustogi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-09 06:52:31 | ID requested | |
⏵ 1 | 2024-10-17 03:07:21 | announced | |
Publication List
10.1093/nar/gkae648; |
Odunze U, Rustogi N, Devine P, Miller L, Pereira S, Vashist S, Snijder HJ, Corkill D, Sabirsh A, Douthwaite J, Bond N, Desai A, RNA encoded peptide barcodes enable efficient in vivo screening of RNA delivery systems. Nucleic Acids Res, 52(16):9384-9396(2024) [pubmed] |
Keyword List
submitter keyword: LNP, proteomics,RNA, in vivo, high throughput, barcodes |
Contact List
Paul Devine |
contact affiliation | Astrazeneca |
contact email | paul.devine@astrazeneca.com |
lab head | |
Nitin Rustogi |
contact affiliation | Astrazeneca |
contact email | nitin.rustogi@astrazeneca.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053797
- Label: PRIDE project
- Name: RNA Encoded Peptide Barcodes Enable Efficient in vivo Screening of RNA Delivery Systems