PXD053788

PXD053788 is an original dataset announced via ProteomeXchange.

Title	Histone monoaminylation dynamics are regulated by a single enzyme and promote neural rhythmicity
Description	Histone H3 monoaminylations at glutamine(Q) 5 represent an important family of epigenetic markers in neurons that play critical roles in the mediation of permissive gene expression (1, 2). We previously demonstrated that H3Q5 serotonylation(ser) and dopaminylation(dop) are catalyzed by the Transglutaminase 2 (TGM2) enzyme and alter both local and global chromatin states (3, 4). Here, we found that TGM2 additionally functions as an “eraser” of H3 monoaminylations that is capable of “re-writing” these epigenetic marks in cells, including a new class of this modification, H3Q5 histaminylation(his), which displays dynamic diurnal expression in brain and contributes to neural rhythmicity. We found that H3Q5his inhibits binding of the MLL1 complex to the H3 N-terminus and attenuates its methyltransferase activity on H3 lysine(K) 4. We determined that H3Q5 monoaminylation dynamics are dictated by local monoamine concentrations, which are utilized by TGM2. Taken together, we present here a novel mechanism through which a single chromatin regulatory enzyme is capable of sensing chemical microenvironments to affect the epigenetic states of cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_07:01:12.234.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	henrik molina
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	5-glutamyl serotonin; N6,N6,N6-trimethyl-L-lysine; N6,N6-dimethyl-L-lysine
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-09 02:07:37	ID requested
1	2024-10-11 05:08:09	announced
⏵ 2	2024-10-22 07:01:12	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: TGM2, bidirectionally controls H3 monoaminylation dynamics, which, facilitate neural rhythmicity., in turn,A single enzyme

ian maze
contact affiliation	Ichan School of Medicine at Mount Sinai
contact email	ian.maze@mssm.edu
lab head
henrik molina
contact affiliation	THE ROCKEFELLER UNIVERSITY
contact email	henrik.molina@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD053788

PRIDE project URI

• PRIDE
  1. PXD053788
     1. Label: PRIDE project
     2. Name: Histone monoaminylation dynamics are regulated by a single enzyme and promote neural rhythmicity