PXD053787

PXD053787 is an original dataset announced via ProteomeXchange.

Title	T Lymphocytes induce the expression of Coronin-1A in breast cancer cells enabling the transmigration through a blood-brain-barrier model
Description	Background: In previous work we discovered that T lymphocytes play a prominent role in the rise of brain metastases of ER-negative breast cancers. In the present study we explored expressional changes due to T cell contact associated with penetration through the BBB for breast cancer cell lines derived from cancers with various affinities for brain. Methods: Differential expression of proteins was identified by comparing the proteomes of the breast cancer cells before and after co-culture with T cells by using liquid chromatography-mass spectrometry (LC-MS). siRNA was used to silence protein expression in the tumor cells and the artificial BBB model was employed to study the effects on passage of the breast carcinoma cell lines. Results: Mass spectrometry-based proteomics revealed significant alterations in the expression of 35 proteins by the breast cancer cell lines upon T cell contact. Among the proteins is coronin-1A, a protein related to cell motility. Knockdown of CORO1A in the breast cancer cells reduced their ability to cross the artificial BBB to 60%. The effects were significantly less for the cell line derived from breast cancer with affinity for brain. The expression of coronin-1A was confirmed by immunohistochemistry and RT-PCR of 52 breast cancer samples of patients with metastasized breast cancers, with and without brain locations. Lastly, CORO1A upregulation was validated in a publicly available mRNA expression database from 204 primary breast cancers with known metastatic sites. Conclusions: We conclude that T lymphocytes trigger cancer cells to express proteins including coronin-1A thereby facilitating their passage through an in vitro BBB. In addition, a prominent role of coronin-1A in the formation of cerebral metastases in breast cancer patients is strongly suggestive by its upregulation in tissue samples of breast cancer patients with brain metastases.
HostingRepository	PRIDE
AnnounceDate	2025-02-24
AnnouncementXML	Submission_2025-02-24_05:01:41.953.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD053787
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lona Zeneyedpour
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-09 01:45:41	ID requested
⏵ 1	2025-02-24 05:01:42	announced

Kros JM, Zeneyedpour L, Pedrosa RMSM, Belcaid Z, Dik WA, Luider TM, Mustafa DAM, T cell induced expression of Coronin-1A facilitates blood-brain barrier transmigration of breast cancer cells. Sci Rep, 14(1):31516(2024) [pubmed]

10.1038/s41598-024-83301-x;

10.6019/PXD053787;

submitter keyword: breast cancer

brain metastasis

blood-brain barrier

proteomics

liquid chromatography-mass spectrometry

coronin-1A

Theo Luider
contact affiliation	Department of Neurology, Laboratory of Neuro‑Oncology, Clinical and Cancer Proteomics, Erasmus University Medical Center
contact email	t.luider@erasmusmc.nl
lab head
Lona Zeneyedpour
contact affiliation	Erasmus medical center
contact email	l.zeneyedpour@erasmusmc.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053787
     1. Label: PRIDE project
     2. Name: T Lymphocytes induce the expression of Coronin-1A in breast cancer cells enabling the transmigration through a blood-brain-barrier model