PXD053785

PXD053785 is an original dataset announced via ProteomeXchange.

Title	The proteome of APP/PS1 mouse amyloid beta oligomers
Description	Amyloid β (Aβ) oligomers are thought to play an important role in the onset and progression of Alzheimer’s disease (AD). Several oligomer-binding proteins have been implicated in mechanisms leading to AD pathology. Such proteins could provide insights into the pathways involved in the disease process of AD and might reveal novel targets for disease-modifying therapy approaches. Here, we separated different native Aβ assemblies in brain homogenates of transgenic AD mice samples by density gradient centrifugation, then isolated Aβ-containing complexes by co-immunoprecipitation. Mass spectrometry of immunoprecipitated proteins with label-free quantification (LFQ) showed numerous significant changes between transgenic AD mice and wildtype mice, confirming familiar binding proteins and also indicating putative new interactors.
HostingRepository	PRIDE
AnnounceDate	2026-04-07
AnnouncementXML	Submission_2026-04-07_08:48:09.471.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-09 00:55:57	ID requested
⏵ 1	2026-04-07 08:48:10	announced

10.1021/acs.biochem.5c00597;

Kass B, Poschmann G, Demir F, Huesgen P, St, ü, hler K, Kutzsche J, Willbold D, The Proteome of Human Amyloid Beta Oligomers. Biochemistry, 65(3):339-353(2026) [pubmed]

submitter keyword: Alzheimer’s disease, co-immunoprecipitation, mass spectrometry, brain homogenates,amyloid beta oligomer binding, APP/PS1 mouse

Gereon Poschmann
contact affiliation	Proteome research Molecular Medicine Heinrich-Heine-University Düsseldorf 40225 Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD053785

PRIDE project URI

• PRIDE
  1. PXD053785
     1. Label: PRIDE project
     2. Name: The proteome of APP/PS1 mouse amyloid beta oligomers