PXD053772 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Multiomic analysis identifies involvement of complement-dependent pathways in outcomes after repetitive mild closed head injury |
| Description | Repetitive mild closed head injury (rmCHI) is a common type of traumatic brain injury and its incidence is increasing, not least due to an increase in the number of sports-related injuries. The underlying neuroimmune mechanisms secondary to trauma that link rmCHI to cognitive decline are not well understood, and the role of complement in this context is largely unexplored. We developed a mouse model of rmCHI and demonstrated complement activation in the brain and cognitive decline, measured 21 days after cessation of injury. These outcomes were accompanied by alterations in local and peripheral immune cell recruitment, together with changes in microglial activation status, as determined by mass cytometry and microscopy. RNAseq and proteomic analysis further revealed major changes in neurodegenerative associated pathways after rmCHI, such as pathways involved neuron development, wound healing and neuronal apoptosis. Complement inhibition initiated after cessation of injury modulated rmCHI-induced changes and protected against cognitive decline. |
| HostingRepository | PRIDE |
| AnnounceDate | 2025-12-01 |
| AnnouncementXML | Submission_2025-11-30_16:58:53.267.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jennifer Bethard |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-07-08 13:13:28 | ID requested | |
| ⏵ 1 | 2025-11-30 16:58:54 | announced | |
Publication List
| 10.1038/s41392-025-02466-7; |
| Mallah K, Krieg C, Hatchell D, Hamouda N, Roof T, Walterhouse S, Toutonji A, Borucki D, Couch C, Hardiman G, Kobeissy F, Guglietta S, Tomlinson S, Targeted complement inhibition ameliorates the pathological and cognitive outcomes in repetitive mild closed head injury. Signal Transduct Target Ther, 10(1):378(2025) [pubmed] |
Keyword List
| submitter keyword: Complement System, Repetitive, RNAseq, Cytof, Traumatic Brain Injury, Closed Head, Proteomics, Microglia |
Contact List
| Silvia Guglietta |
|---|
| contact affiliation | Medical University of South Carolina, Department of Regenerative Medicine & Cell Biology, Charleston, SC, USA Hollings Cancer Center, Charleston, SC, USA |
| contact email | gugliett@musc.edu |
| lab head | |
| Jennifer Bethard |
|---|
| contact affiliation | Medical University of SC |
| contact email | bethard@musc.edu |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD053772 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053772
- Label: PRIDE project
- Name: Multiomic analysis identifies involvement of complement-dependent pathways in outcomes after repetitive mild closed head injury
