PXD053768

PXD053768 is an original dataset announced via ProteomeXchange.

Title	The Aryl hydrocarbon receptor and FOS mediated cytotoxicity induced by Acenatobacter baumannii
Description	Acinetobacter baumannii is a highly pathogenic and multidrug-resistant Gram-negative bacterium that causes severe nosocomial infections. To better understand the mechanism of pathogenesis, we compare the proteomes of uninfected and infected human cells, revealing that transcription factor FOS is the host protein most strongly induced by A. baumannii infection. Pharmacological inhibition of FOS reduces the cytotoxicity of A. baumannii in cell-based models, and similar results are also observed in a mouse infection model. A. baumannii outer membrane vesicles (OMVs) are shown to activate the aryl hydrocarbon receptor (AHR) of host cells by inducing the host enzyme tryptophan-2,3-dioxygenase (TDO), producing the ligand kynurenine, which binds AHR. Following ligand binding, AHR is a direct transcriptional activator of the FOS gene. Our results suggest that bacterial infection interacts with host tryptophan metabolism and the transcription factor FOS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:52:29.076.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anshu Bhattacharya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 10:51:32	ID requested
1	2024-08-04 20:28:09	announced
⏵ 2	2024-10-22 06:52:29	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: OMV, Proteome, A549,Acenatobacter Baumannii

Ivan Dikic
contact affiliation	Professor at Goethe University Frankfurt, Director of Institute of Biochemistry 2 and a fellow of Max Planck Society.
contact email	dikic@biochem2.uni-frankfurt.de
lab head
Anshu Bhattacharya
contact affiliation	Denali Therapeutics
contact email	anshu.bhattacharya312@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD053768

PRIDE project URI

• PRIDE
  1. PXD053768
     1. Label: PRIDE project
     2. Name: The Aryl hydrocarbon receptor and FOS mediated cytotoxicity induced by Acenatobacter baumannii