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PXD053767

PXD053767 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitative LC-MS/MS analysis of secretome of Mesenchymal Cells from Tooth Germ of Mice
DescriptionLabel free: The aim of project is to identify the proteins that may have a role in the inductive signal of mesenchymal cells.   Protein identification and quantitively compare the proteins from the following samples: 1- Exosomes purified from embryonic mesenchymal cells of tooth germ after overnight culture in 2D and 3D conditions 2- Secretome of embryonic mesenchymal cells of tooth germ after overnight 3D culture. 3- Embryonic mesenchymal cells from tooth germ- fresh frozen as pellet TMT Samples 10.5 and 14.5 are exosomes derived from immortalized cell line and different stages of tooth embryonic development. These sets of samples were seeded in well plate. The embryonic samples are a pool of 6 biological replicates. The aim is to identify the different proteins between these stages as well as the cell line.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-06_21:29:42.784.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSteven Lynham
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-08 10:37:42ID requested
12025-05-06 21:29:43announced
Publication List
10.3390/bioengineering12020096;
Birjandi AA, Sharpe P, The Secretome of the Inductive Tooth Germ Exhibits Signals Required for Tooth Development. Bioengineering (Basel), 12(2):(2025) [pubmed]
Keyword List
submitter keyword: Tandem Mass Tagging, Mouse,Secretome, Orbitrap Fusion Lumos, LC-MS/MS
Contact List
Prof. Paul Sharpe
contact affiliationCentre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, Guy's Hospital, Floor 27, Tower Wing, London, SE1 9RT, United Kingdom
contact emailpaul.sharpe@kcl.ac.uk
lab head
Steven Lynham
contact affiliationKing's College London
contact emailsteven.lynham@kcl.ac.uk
dataset submitter
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Dataset FTP location
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