PXD053767 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative LC-MS/MS analysis of secretome of Mesenchymal Cells from Tooth Germ of Mice |
Description | Label free: The aim of project is to identify the proteins that may have a role in the inductive signal of mesenchymal cells. Protein identification and quantitively compare the proteins from the following samples: 1- Exosomes purified from embryonic mesenchymal cells of tooth germ after overnight culture in 2D and 3D conditions 2- Secretome of embryonic mesenchymal cells of tooth germ after overnight 3D culture. 3- Embryonic mesenchymal cells from tooth germ- fresh frozen as pellet TMT Samples 10.5 and 14.5 are exosomes derived from immortalized cell line and different stages of tooth embryonic development. These sets of samples were seeded in well plate. The embryonic samples are a pool of 6 biological replicates. The aim is to identify the different proteins between these stages as well as the cell line. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-06_21:29:42.784.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Steven Lynham |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-08 10:37:42 | ID requested | |
⏵ 1 | 2025-05-06 21:29:43 | announced | |
Publication List
10.3390/bioengineering12020096; |
Birjandi AA, Sharpe P, The Secretome of the Inductive Tooth Germ Exhibits Signals Required for Tooth Development. Bioengineering (Basel), 12(2):(2025) [pubmed] |
Keyword List
submitter keyword: Tandem Mass Tagging, Mouse,Secretome, Orbitrap Fusion Lumos, LC-MS/MS |
Contact List
Prof. Paul Sharpe |
contact affiliation | Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, Guy's Hospital, Floor 27, Tower Wing, London, SE1 9RT, United Kingdom |
contact email | paul.sharpe@kcl.ac.uk |
lab head | |
Steven Lynham |
contact affiliation | King's College London |
contact email | steven.lynham@kcl.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053767 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD053767
- Label: PRIDE project
- Name: Quantitative LC-MS/MS analysis of secretome of Mesenchymal Cells from Tooth Germ of Mice