PXD053767

PXD053767 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative LC-MS/MS analysis of secretome of Mesenchymal Cells from Tooth Germ of Mice
Description	Label free: The aim of project is to identify the proteins that may have a role in the inductive signal of mesenchymal cells. Protein identification and quantitively compare the proteins from the following samples: 1- Exosomes purified from embryonic mesenchymal cells of tooth germ after overnight culture in 2D and 3D conditions 2- Secretome of embryonic mesenchymal cells of tooth germ after overnight 3D culture. 3- Embryonic mesenchymal cells from tooth germ- fresh frozen as pellet TMT Samples 10.5 and 14.5 are exosomes derived from immortalized cell line and different stages of tooth embryonic development. These sets of samples were seeded in well plate. The embryonic samples are a pool of 6 biological replicates. The aim is to identify the different proteins between these stages as well as the cell line.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_21:29:42.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Steven Lynham
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 10:37:42	ID requested
⏵ 1	2025-05-06 21:29:43	announced

Publication List

10.3390/bioengineering12020096;
Birjandi AA, Sharpe P, The Secretome of the Inductive Tooth Germ Exhibits Signals Required for Tooth Development. Bioengineering (Basel), 12(2):(2025) [pubmed]

10.3390/bioengineering12020096;

Birjandi AA, Sharpe P, The Secretome of the Inductive Tooth Germ Exhibits Signals Required for Tooth Development. Bioengineering (Basel), 12(2):(2025) [pubmed]

Keyword List

submitter keyword: Tandem Mass Tagging, Mouse,Secretome, Orbitrap Fusion Lumos, LC-MS/MS

submitter keyword: Tandem Mass Tagging, Mouse,Secretome, Orbitrap Fusion Lumos, LC-MS/MS

Contact List

Prof. Paul Sharpe
contact affiliation	Centre for Craniofacial and Regenerative Biology, Faculty of Dentistry, Oral & Craniofacial Sciences, Guy's Hospital, Floor 27, Tower Wing, London, SE1 9RT, United Kingdom
contact email	paul.sharpe@kcl.ac.uk
lab head
Steven Lynham
contact affiliation	King's College London
contact email	steven.lynham@kcl.ac.uk
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053767
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD053767

PRIDE project URI

Repository Record List

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