PXD053764

PXD053764 is an original dataset announced via ProteomeXchange.

Title	The kinase PknB modulates the cell wall biosynthetic machinery
Description	Streptomyces are filamentous bacteria living mostly in the soil and best known for their ability to produce specialised metabolites (e.g. antibiotics). They grow as tip-extending, branching hyphal filaments to form a multicellular mycelium. New branches are established by the formation of a new growth zone on the lateral cell wall. Proteins responsible for this process are organised in complexes called polarisomes, with DivIVA being the best described component. We have developed a genetic screen based on DivIVA overexpression to identify proteins potentially interacting with DivIVA in Streptomyces albus. Among the hits was the important morphogenic genes encoding PknB (Ser/Thr kinase). We confirmed that deletion of pknB rescues S. albus from the effects of overexpressing DivIVA. Next, we studied the impact of pknB deletion in Streptomyces. A mass spectrometry phosphoproteome analysis indicated that absence of PknB alters the phosphorylation state of CslA, a cellulose synthase-like protein, containing a transglycosylase domain. Our research demonstrated that a phosphoablative mutant of CslA impacts its activity in β-glucan synthesis, as it displays a similar hypersensitivity to lysozyme as a CslA deletion strain. This highlights one pathway through which PknB affects the stability of the cell wall structure and possibly explains its impact in rescuing the cell from a lethal overproduction of DivIVA. Our findings highlight the role of PknB in maintaining the cellular structure and integrity of the Streptomyces cell wall.
HostingRepository	PRIDE
AnnounceDate	2026-09-07
AnnouncementXML	Submission_2026-09-06_16:37:54.084.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthias Trost
SpeciesList	scientific name: Streptomyces albus; NCBI TaxID: NEWT:1888; scientific name: Bacteria; NCBI TaxID: NCBITaxon:2;
ModificationList	phosphorylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 08:55:40	ID requested
⏵ 1	2026-09-06 16:37:54	announced

10.1111/mmi.70086;

Derkacz M, Watson A, Gajbhiye A, Tracz M, Jakimowicz D, Trost M, Errington J, Kepplinger B, Roles of PknB and CslA in Cell Wall Morphogenesis of Streptomyces. Mol Microbiol, 126(2):161-172(2026) [pubmed]

submitter keyword: Streptomyces albus

Matthias Trost
contact affiliation	Newcastle University Biosciences Institute, Faculty of Medical Sciences, Newcastle upon Tyne, NE2 4HH, UK
contact email	matthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliation	Newcastle University
contact email	matthias.trost@ncl.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053764
     1. Label: PRIDE project
     2. Name: The kinase PknB modulates the cell wall biosynthetic machinery