PXD053759

PXD053759 is an original dataset announced via ProteomeXchange.

Title	The proteome of human amyloid beta oligomers
Description	Amyloid β (Aβ) oligomers are thought to play an important role in the onset and progression of Alzheimer’s disease (AD). Several oligomer-binding proteins have been implicated in mechanisms leading to AD pathology. Such proteins could provide insights into the pathways involved in the disease process of AD and might reveal novel targets for disease-modifying therapy approaches. Here, we separated different native Aβ assemblies in brain homogenates of human AD post mortem tissue by density gradient centrifugation, then isolated Aβ-containing complexes by co-immunoprecipitation. Mass spectrometry of immunoprecipitated proteins with label-free quantification (LFQ) showed numerous significant changes between AD and control samples, confirming familiar binding proteins and also indicating putative new interactors.
HostingRepository	PRIDE
AnnounceDate	2026-04-07
AnnouncementXML	Submission_2026-04-07_09:24:26.137.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD053759
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-08 08:04:27	ID requested
⏵ 1	2026-04-07 09:24:27	announced

10.1021/acs.biochem.5c00597;

10.6019/PXD053759;

submitter keyword: Alzheimer’s disease, co-immunoprecipitation, mass spectrometry, brain homogenates,amyloid beta oligomer binding

Gereon Poschmann
contact affiliation	Proteome research Molecular Medicine Heinrich-Heine-University Düsseldorf 40225 Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053759
     1. Label: PRIDE project
     2. Name: The proteome of human amyloid beta oligomers