PXD053701

PXD053701 is an original dataset announced via ProteomeXchange.

Title	Biocompatible 'ligand balancing' in transition metal coordination allows benign in-cell protein arylation - 90 min 1f-labelling EXPT - Fragpipe offset search for selectivity
Description	organonickel-mediated S arylation tolerates flexible chelation with biocompatible ligands without ablating the chemical reactivity of corresponding aryl nickel reagents. This, critically, now allows the creation of safe, site-selective C–S-bond-forming arylation manifolds that mimic nature’s use of balanced ligand state. These prove sufficiently benign for use not only on diverse protein substrates in vitro but even on the surface of and inside living prokaryotic and eukaryotic cells. This, in turn, now allows the deepest chemical surveys of reactive cysteines in cells known to date, several-fold outstripping traditional organic reagents (e.g. ~11,000 in human embryonic kidney).
HostingRepository	PRIDE
AnnounceDate	2025-12-10
AnnouncementXML	Submission_2025-12-10_02:23:16.144.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weibing Liu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Ascend

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-05 09:42:28	ID requested
⏵ 1	2025-12-10 02:23:16	announced

10.1038/S41557-025-02017-1;

submitter keyword: Cysteinome profiling, Organonickel-mediated S arylation,ligand balancing

Benjamin G.
contact affiliation	The Rosalind Franklin Institute, Oxfordshire, OX11 0FA, UK Department of Pharmacology, University of Oxford, OX1 3QT, UK Department of Chemistry, University of Oxford, OX1 3TA, UK
contact email	Ben.Davis@rfi.ac.uk
lab head
Weibing Liu
contact affiliation	The Rosalind Franklin Institute, Oxfordshire, OX11 0FA, UK Department of Pharmacology, University of Oxford, OX1 3QT, UK
contact email	weibingliu@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD053701

PRIDE project URI

• PRIDE
  1. PXD053701
     1. Label: PRIDE project
     2. Name: Biocompatible 'ligand balancing' in transition metal coordination allows benign in-cell protein arylation - 90 min 1f-labelling EXPT - Fragpipe offset search for selectivity