PXD053698

PXD053698 is an original dataset announced via ProteomeXchange.

Title	Mouse CSF analysis of 3, 6, and 12 months old mice in comparison to human CSF
Description	Mice are the most commonly used preclinical animal model, but protein analytics of murine cerebrospinal fluid (CSF) remains challenging because of low CSF volume (often <10 µl) and frequent blood contaminations. We developed an improved CSF sampling method that allows routine collection of increased volumes (20-30 µl) of pure CSF from individual mice, enabling multiple protein analytical assays from a single sample. Here, we have analysed the CSF of 3, 6, and 12 months old wild-type mice. Additionally, we collected murine CSF of the same mice at 3 and 6 months of age to compare repeated and single CSF sampling. Furthermore, we analysed human CSF samples as a reference.
HostingRepository	PRIDE
AnnounceDate	2025-04-15
AnnouncementXML	Submission_2025-04-15_09:27:39.011.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-05 07:15:14	ID requested
⏵ 1	2025-04-15 09:27:39	announced

Lourbopoulos A, M, ü, ller SA, Jocher G, Wick M, Plesnila N, Lichtenthaler SF, An Improved Method for Sampling and Quantitative Protein Analytics of Cerebrospinal Fluid of Individual Mice. Mol Cell Proteomics, 24(5):100958(2025) [pubmed]

10.1016/j.mcpro.2025.100958;

submitter keyword: cerebrospinal fluid, CSF, mouse,human

Stefan F. Lichtenthaler
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD053698

PRIDE project URI

• PRIDE
  1. PXD053698
     1. Label: PRIDE project
     2. Name: Mouse CSF analysis of 3, 6, and 12 months old mice in comparison to human CSF