PXD053697

PXD053697 is an original dataset announced via ProteomeXchange.

Title	Proteasome-dependent protein degradation shapes developmental tempo in mouse and human neural progenitors
Description	Developmental programs are conserved across mammals, but the developmental pace varies between species. Stem cell models have revealed that human neural progenitors develop more slowly than the mouse counterpart due to reduced protein degradation rates. Key questions remain about species-specific protein half-lives, its impact on overall protein levels, and the regulation of protein production and degradation during development. The effect of manipulating individual protein stability on developmental pace is also unclear. Here we analyzed protein production, degradation, and abundance in mouse and human neural progenitors using targeted protein labeling, proteomics, and functional genomics. We identified species differences in protein turnover and found that both active degradation influence tempo divergence between mouse and human. Moreover, proteolytic degradation of a key regulatory protein was shown to affect developmental pace. Our study stresses the role of protein degradation in controlling developmental tempo, with implications for phenotypic diversity across evolution.
HostingRepository	PRIDE
AnnounceDate	2026-07-14
AnnouncementXML	Submission_2026-07-14_11:22:14.272.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	David Oxley
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606; scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-05 06:59:01	ID requested
⏵ 1	2026-07-14 11:22:14	announced

Dataset with its publication pending

submitter keyword: development, protein turnover, pulse SILAC

david oxley
contact affiliation	babraham institute
contact email	david.oxley@babraham.ac.uk
lab head
David Oxley
contact affiliation	Babraham Institute
contact email	david.oxley@babraham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053697
     1. Label: PRIDE project
     2. Name: Proteasome-dependent protein degradation shapes developmental tempo in mouse and human neural progenitors