PXD053678

PXD053678 is an original dataset announced via ProteomeXchange.

Title	High-throughput proteomic and phosphoproteomic approaches revealing the salt-responsive mechanisms in two different rice varieties
Description	Salinity stress induces ionic and osmotic imbalances in rice plants that in turn negatively affect photosynthesis rate, resulting in growth retardation and yield penalty. Efforts have, therefore, been carried out to understand the mechanism of salt tolerance, however, the complexity of biological processes at proteome levels remains a major challenge. Here, we performed a comparative proteome and phosphoproteome profiling of microsome enriched fractions of salt-tolerant (cv. IR73; indica) and salt-susceptible (cv. Donjin; japonica) rice varieties. This approach led to the identification of 5,856 proteins, of which 473 and 484 proteins showed differential modulation between DJ and IR sample sets, respectively. The phosphoproteome analysis led to the identification of a total 10,873 phosphopeptides of these 2,929 and 3,049 significant phosphopeptides were identified in DJ and IR sample sets, respectively. The integration of proteome and phosphoproteome data showed activation of ABA and Ca2+ signaling components exclusively in IR in response to salt stress. Taken together, our results highlight the changes at proteome and phosphoproteome levels and provide a mechanistic understand of salt stress tolerance in rice.
HostingRepository	PRIDE
AnnounceDate	2024-12-16
AnnouncementXML	Submission_2024-12-16_05:41:23.258.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa Indica Group x Oryza sativa Japonica Group; NCBI TaxID: 1050722;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-05 01:47:19	ID requested
⏵ 1	2024-12-16 05:41:24	announced

Min CW, Gupta R, Lee GH, Cho JH, Kim YJ, Wang Y, Jung KH, Kim ST, Integrative Proteomic and Phosphoproteomic Profiling Reveals the Salt-Responsive Mechanisms in Two Rice Varieties (Oryza Sativa subsp. Japonica and Indica). Proteomics, ():e202400251(2024) [pubmed]

10.1002/pmic.202400251;

submitter keyword: Oryza sativa,Salinity-stress, Calcium signaling, Proteome, MME, Indica subspecies, Phosphoproteome, Tolerance

Sun Tae Kim
contact affiliation	Department of Plant Bioscience, Life and Industry Convergence Research Institute, Pusan National University, Miryang 50463, Republic of Korea
contact email	stkim71@pusan.ac.kr
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD053678
     1. Label: PRIDE project
     2. Name: High-throughput proteomic and phosphoproteomic approaches revealing the salt-responsive mechanisms in two different rice varieties